Culture medium for vibrio BS02 and preparation method thereof

A kind of culture medium, technology of Vibrio, applied in the culture medium of Vibrio BS02 and its preparation field
CN102154181AInactive Publication Date: 2011-08-17XIAMEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
XIAMEN UNIV
Publication Date
2011-08-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a culture medium for vibrio BS02 and a preparation method thereof, relating to a microbial culture medium and providing a culture medium which is suitable for culture of the vibrio BS02 and can obviously increase cell live weight of the vibrio BS02 and yield of algae-killing active substances. The preparation method comprises the steps of: adding tryptone, yeast powder, glucose, NaCl, MgSO4.7H2O, KCl, K2HPO4.3H2O, CaCl2.2H2O and Fe2(SO4)3 into distilled water, enabling a constant volume to be 1L, and sterilizing to obtain the culture medium of the vibrio BS02. A composite culture medium suitable to high-dense culture of the vibrio BS02 is developed by taking an LB (Luria-Bertani) culture medium as a basic culture medium, investigating the influence of the components of the culture medium and the addition amount thereof to shaking culture of the vibrio BS02, and optimizing the culture medium by utilizing a Plackett-Burman (PB) design method and a response surface optimized design method.
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Description

technical field

[0001] The invention relates to a microorganism culture medium, in particular to a culture medium of Vibrio BS02 and a preparation method thereof. Background technique

[0002] With the rapid development of modern industrialization and the increase of population, a large amount of industrial and agricultural wastewater and domestic sewage are directly discharged into the ocean without any treatment, causing the eutrophication of marine water to intensify, and red tides frequently occur (1, Hallegraeff GM. A review of harmful algal blooms and their apparent global increase [J]. Phycologia. 1993, 32: 79-99). Harmful red tide has become a global marine disaster, seriously restricting the development of coastal economy, destroying the marine ecological environment and threatening human health. Therefore, how to effectively prevent and control red tide has become an urgent problem at home and abroad.

[0003] In the marine ecosystem, microorganisms play an import...

Claims

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