Production method of non-denatured II-type collagen

A collagen and production method technology, applied in the field of collagen, can solve problems such as cumbersome process conditions and unfavorable large-scale production, and achieve the effect of simple extraction process, simple and easy technical solution, and less human factors

Active Publication Date: 2013-05-01
北京华达杰瑞生物技术有限公司
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  • Abstract
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Problems solved by technology

However, the technology of macromolecular collagen with a complete collagen structure is mostly limited to the laboratory scale, because the laboratory scale can meet the tedious and harsh process conditions, but it is not conducive to large-scale production

Method used

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  • Production method of non-denatured II-type collagen

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Embodiment Construction

[0022] Such as figure 1 Shown is the production flow chart of the present invention, below with reference to accompanying drawing and preferred embodiment, technical scheme of the present invention is described in detail.

[0023] 1. Obtain cartilage materials that meet the needs.

[0024] The raw material is taken from animal cartilage, such as pig articular cartilage, chicken articular cartilage, chicken breast cartilage. After the raw materials are obtained, the raw materials are usually not used directly, and there are often impurities such as residual meat, perichondrium, and calcified blocks remaining on them. Manual removal is time-consuming and labor-intensive, which increases the cost. In the present invention, the ratio of ProteaseN: pepsin = 1:16 (w / w) is used to stir and hydrolyze at 26°C to 28°C for 24 hours under the environment of pH 6.5 and pH 2.5 respectively. As a result, impurities are basically removed and the morphology Intact, cartilage material that me...

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Abstract

The invention discloses a production method of non-denatured II-type collagen, which comprises the steps of: during removal of impurities, stirring and hydrolyzing 1:16w / w pepsase for 24h in the temperature of 26-28 DEG C respective under pH 6.5 and pH2.5 conditions, obtaining cartilage material and then grinding the obtained cartilage material into powder; and extracting collagen, and obtaining the non-denatured II-type collagen. The production method leads the extraction of the non-denatured collagen to be expanded into production scale from laboratory scale, thus basically meeting the production requirements; the technical scheme of the invention is simple and convenient to do, strong in standardization, less in human factors, cost-saving and high in yield; and the extraction process of the collagen is simpler and more convenient, so that the large-scale production can be more smoothly carried out.

Description

technical field [0001] The invention relates to collagen technology, in particular to a method for producing non-denatured type II collagen. Background technique [0002] Collagen is the extracellular matrix. The earliest people paid attention to the impact of cells on life activities. Through research, it was found that the extracellular matrix is ​​also very important to life. In the past ten years, it has been discovered that the extracellular matrix, especially collagen, protein Polysaccharides play a key role in human health and the occurrence of certain diseases. The medical and health care products markets are also increasingly developing collagen-related products. [0003] Collagen is a large family, it only exists in animals, there is no collagen in plants. This is an important principle. In animals, collagen is distributed in bones, cartilage, skin, fascia, etc. Collagen is different from the double helix structure of other proteins, but a triple helix structure...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/14
Inventor 李洁朱大开龚辉
Owner 北京华达杰瑞生物技术有限公司
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