Red flower orange daylily tissue culture formula and culture method
A technology for daylilies and safflowers, applied in the field of tissue culture of daylilies, can solve problems such as poor consistency of plant type, large plant variability, and inability to maintain excellent traits, and achieve short cycle, stable production system, and large-scale rapid propagation The effect of production
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[0033] The present invention will be further described below in conjunction with specific embodiment:
[0034] combine figure 1 , first, sow the seeds of Hemerocallis safflower 'Ruby' to obtain a certain amount of sowing seedlings. The amount of sowing seedlings obtained in the present embodiment is about 500, and collect robust flowers, stem nodes, tubers, receptacles and other tissues without damage by diseases and insect pests. Organs, as explants in tissue culture. Mix the collected explants with washing powder and rinse them under running water for 30 minutes, mix them with washing powder equal to the weight of the explants, disinfect them with 75% alcohol for 10-30s on the ultra-clean workbench, and then wash them with sterile water Rinse 3-5 times, then disinfect with 0.1% mercuric chloride for 2-20 minutes, and select the best explant disinfection method: 0.1% (mass percentage) mercuric chloride for 10 minutes.
[0035] Second, inoculate the sterilized explants on th...
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