Red flower orange daylily tissue culture formula and culture method

A technology for daylilies and safflowers, applied in the field of tissue culture of daylilies, can solve problems such as poor consistency of plant type, large plant variability, and inability to maintain excellent traits, and achieve short cycle, stable production system, and large-scale rapid propagation The effect of production

Inactive Publication Date: 2011-09-07
HEILONGJIANG VOCATIONAL INST OF ECOLOGICAL ENG
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  • Claims
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Problems solved by technology

[0003] Hemerocallis is often propagated by seeds, and can also be propagated by division and cuttings. However, the traditional method of propagation has poor consistency of plant type, large plant variability, and excellent traits cannot be mainta...

Method used

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  • Red flower orange daylily tissue culture formula and culture method
  • Red flower orange daylily tissue culture formula and culture method

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Embodiment Construction

[0033] The present invention will be further described below in conjunction with specific embodiment:

[0034] combine figure 1 , first, sow the seeds of Hemerocallis safflower 'Ruby' to obtain a certain amount of sowing seedlings. The amount of sowing seedlings obtained in the present embodiment is about 500, and collect robust flowers, stem nodes, tubers, receptacles and other tissues without damage by diseases and insect pests. Organs, as explants in tissue culture. Mix the collected explants with washing powder and rinse them under running water for 30 minutes, mix them with washing powder equal to the weight of the explants, disinfect them with 75% alcohol for 10-30s on the ultra-clean workbench, and then wash them with sterile water Rinse 3-5 times, then disinfect with 0.1% mercuric chloride for 2-20 minutes, and select the best explant disinfection method: 0.1% (mass percentage) mercuric chloride for 10 minutes.

[0035] Second, inoculate the sterilized explants on th...

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Abstract

The invention provides a red flower orange daylily tissue culture formula and a culture method. The method comprises the following steps: disinfecting an explant to obtain an aseptic line; using proportioning of exogenous hormone of different types and concentrations to carry out sub-proliferation on tissue culture seedlings and promoting growth thereof, screening and regulating rootedness; and finally, determining the best explant and culture medium formula for promoting application of the explant and the formula in industrialized production.The tissue culture formula provided by the invention can be employed to substantially expand proliferation of the red flower orange daylily 'Ruby' and fast and substantially reproduce tissue culture seedlings to meet the demands of landscaping.The method provided by the invention is remarkably superior to tissue culture and rapid propagation of the previous yellow orange daylily products in terms of disinfection time and effect, operation methods, regeneration efficiency, production costs and the like. The large-scale production system based on the method is stable in operation and has short production cycle, thus being capable of meeting large-scale rapid propagation.

Description

(1) Technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a tissue culture technique of Hemerocallis safflower. (2) Background technology [0002] Hemerocallis is a perennial herbaceous perennial flower of Liliaceae, native to my country, Japan and southern Europe. The color is gorgeous, and it is widely used in urban greening and garden landscape construction. There are cultivars for all flower colors except blue and pure white. The flower diameter varies greatly, and the flowering period is from late spring to autumn, and the flowering period of most varieties is weeks or even months. Generally, asexual reproduction methods such as branching and stem bud cutting are used for propagation. Due to its slow speed, generally one plant of Hemerocallis can only reproduce 4 to 5 plants per year, so it is difficult to meet the needs of commercial production in the market. The leaves of Hemerocallis safflower are narrow and lon...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 卢宝伟孟庆敏安凤霞王洪军郑宝仁唐翠景向欣徐国柱黄石竹李伟苏小霞
Owner HEILONGJIANG VOCATIONAL INST OF ECOLOGICAL ENG
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