Method for detecting water toxicity by using biologic fish toxicity test

A toxicity test, biological technology, applied in the direction of biological testing, water testing, application, etc., to achieve the effect of reducing the risk of human cancer

Inactive Publication Date: 2011-09-07
济南市供排水监测中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problem that the traditional biological fish experiment method cannot clearly and clearly reflect the toxicity of water quality, the invention adopts the toxic substance chromium as a standard poison, and reflects the toxicity of water quality with the concentration of chromium ions, so that the quantitative evaluation of water quality toxicity can be realized

Method used

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  • Method for detecting water toxicity by using biologic fish toxicity test
  • Method for detecting water toxicity by using biologic fish toxicity test
  • Method for detecting water toxicity by using biologic fish toxicity test

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Toxicity testing was carried out on the surface water containing 0.04mg / L deltamethrin in this city. The water quality conditions of the surface water were: pH 8.07, temperature 25°C, dissolved oxygen 9mg / L, hardness 305 mg / L.

[0033] The detection steps are:

[0034] (1) Select medaka fish (Medakafish, Oryzias latipes) with a body length between 2 and 3 cm, and put them in standard water with the same water quality conditions (the standard water is prepared with distilled water or deionized water, and its water quality conditions are the same as those of surface water Consistent) for 7 days of domestication. During the domestication period, the mortality rate of medaka fish was guaranteed to be lower than 10%. If it was higher than 10%, a new batch of fish was domesticated again. Fasting began 1 day before the test;

[0035] (2) in Cr 6+ Within the concentration range of 0.5-50 mg / L, 6 concentration points were selected for biological fish toxicity test respectively...

Embodiment 2

[0040] The tap water in this city added 0.0625 mg / L ferric ions was tested for toxicity. The water quality conditions of the surface water are: pH 8.15, temperature 25°C, dissolved oxygen 10 mg / L, and hardness 298 mg / L.

[0041] The detection steps are:

[0042](1) Select medaka fish (Medakafish, Oryzias latipes) with a body length between 2 and 3 cm, and put them in standard water with the same water quality conditions (the standard water is prepared with distilled water or deionized water, and its water quality conditions are the same as those of surface water Consistent) for 7 days of domestication. During the domestication period, the mortality rate of medaka fish was guaranteed to be lower than 10%. If it was higher than 10%, a new batch of fish was domesticated again. Fasting began 1 day before the test;

[0043] (2) in Cr 6+ Within the concentration range of 0.5-50 mg / L, 6 concentration points were selected for biological fish toxicity test respectively. The chromium ...

Embodiment 3

[0048] Test according to the method of embodiment 1, difference is, the medaka fish used during test is 20 tails.

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Abstract

The invention discloses a method for detecting water toxicity by using a biologic fish toxicity test. The method comprises the steps of: breeding medakas; preparing Cr6+ solutions at different concentrations; performing the biologic fish toxicity test by taking standard water as a control group and Cr6+ solutions at different concentrations as test groups; detecting death rates of medakas after 24 hours, 48 hours, 78 hours and 96 hours; drawing a standard curve of Cr6+ to the death rate; performing the biologic fish toxicity test by using a water sample to be detected and setting up a standard water control group; detecting the death rates of medakas after 24 hours, 48 hours, 78 hours and 96 hours; substituting the corresponding standard curve; and representing the water toxicity of the water sample with Cr6+ concentration. Through the standard curve of Cr6+ to the death rate, the water toxicity is quantitatively evaluated and the water pollution condition can be judged; the method provides technical supports for sudden water pollution and a water treatment technology of a water plant; and the risk of causing human cancers by exposure of drinking water is reduced.

Description

technical field [0001] The invention relates to a method for detecting water quality toxicity by adopting a biological fish toxicity test, in particular to a method for detecting water quality toxicity by a quantitative biological fish toxicity test. Background technique [0002] In order to ensure drinking water safety and reduce the threat of environmental degradation to drinking water quality, people often use various methods to evaluate water quality to effectively grasp the health risks caused by drinking water. [0003] The biological fish toxicity test is one of the classic comprehensive biological toxicity evaluation methods, including the acute toxicity test of fish and the test of analyzing its physiological and biochemical indicators with fish as the carrier. By observing the symptoms of fish poisoning at any time and recording the behavior, physiology, biochemical Characteristics and other indicators to grasp the pollution status of water bodies. The fish acute ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/18G01N33/50A01K61/00
CPCY02A40/81
Inventor 贾瑞宝宋艳孙韶华
Owner 济南市供排水监测中心
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