Device and method for sorting cells

A cell and sorting technology, which is applied in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve the problems of inability to realize the cell sorting function and complicated operation of the sorting process, and achieve good results. Effects of Optical Properties and Chemical Stability

Inactive Publication Date: 2011-09-14
HARBIN INST OF TECH AT WEIHAI
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  • Abstract
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  • Claims
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Problems solved by technology

The entire sorting process of this method is complicated to operate. When the surface of the cells to be sorted does not contain antigens...

Method used

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  • Device and method for sorting cells
  • Device and method for sorting cells

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Embodiment Construction

[0019] The present invention will be further described below in conjunction with the accompanying drawings.

[0020] A cell sorting device is provided with a microfluidic chip, and the microfluidic chip is provided with a cell sorting chamber 6 for storing mixed cells and several cell collecting chambers. In this embodiment, three cell collection chambers 11, 12, 13 are provided for collecting three different types of cells. The cell sorting chamber 6 and each cell collection chamber 11, 12, 13 are respectively connected through the middle microfluidic channels 8, 9, 10; the side of the cell sorting chamber 6 is provided with a side microfluidic channel 7 leading to the outside of the chip, and through The capillary is connected with the culture fluid pumping device 4; the rear part of the cell sorting chamber 6 is provided with a rear microfluidic channel 14, and is connected with the mixing cell pumping device 5 through the capillary; the cell collecting chambers 11, 12, 13 ...

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Abstract

The invention relates to a device and method for sorting cells. In the device, a micro-fluidic chip is used as a core; after a sterile capillary and a medical extraction and injection device are connected to the outer side of the device, a fully enclosed sterile environment is formed in the micro-fluidic chip; mixed cell culture solution is injected into a cell sorting chamber in the micro-fluidic chip by using the extraction and injection device; cells to be sorted are captured in the cell sorting chamber by using femtosecond optical tweezers and are moved into a cell collection chamber; andafter a sufficient number of cells are stored in the cell collection chamber, cells in the cell collection chamber are injected into the sterile capillary by injecting the sterile culture solution into the cell sorting chamber, and the capillary is taken off, closed and stored, so that an entire cell sorting operation process is finished. In the cell sorting operation process, the mixed cell culture solution is isolated from the exterior, and the cell culture solution is prevented from being polluted. The device has the advantages of reasonable structure, easiness for manufacturing, convenience for sorting operation and high success rate and can be applied to the field of life science.

Description

technical field [0001] The invention relates to a device and method for sorting cells, in particular to a cell sorting method using a microfluidic chip as a cell sorting container, and using femtosecond optical tweezers in the microfluidic chip to sort cells. technical background [0002] Cells are the basic unit of life activities, and the answers to all life problems can be found in cells. The development of cell biology relies heavily on the manipulation of single living cells. In genetic engineering, bacteria containing special gene fragments are marked with fluorescent genes. Not all bacteria will be successfully marked during the fluorescent labeling process, so it is necessary to sort out the successfully marked bacteria and culture them separately; in addition, after the marked bacteria proliferate In order to detect whether the gene fragment can be stably expressed after the bacteria have been subcultured, it is still necessary to sort out the bacteria that contain...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12N5/00C12N1/20
CPCC12M23/16C12M47/04
Inventor 曲士良李岩
Owner HARBIN INST OF TECH AT WEIHAI
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