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Kit for specific PCR (polymerase chain reaction) detection of pneumocystis and detection method thereof

A detection kit and detection method technology, applied in the field of biomedicine, can solve the problems of early diagnosis of uncomfortable diseases and little significance of clinical diagnosis and treatment, and achieve the effects of rigorous design, high sensitivity and strong specificity

Inactive Publication Date: 2011-09-14
苏州深久医药生物技术有限公司
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  • Application Information

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Problems solved by technology

However, this method is not suitable for the early diagnosis of the disease, and has little significance in clinical diagnosis and treatment.

Method used

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  • Kit for specific PCR (polymerase chain reaction) detection of pneumocystis and detection method thereof
  • Kit for specific PCR (polymerase chain reaction) detection of pneumocystis and detection method thereof
  • Kit for specific PCR (polymerase chain reaction) detection of pneumocystis and detection method thereof

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Embodiment Construction

[0057] The invention discloses a specific PCR detection kit for Pneumocystis sp. and a detection method thereof.

[0058] Below in conjunction with embodiment detailed further description:

[0059] (1) Pneumocystis specific diagnostic sequence:

[0060] Pneumocystis mitochondrial large subunit rRNA gene, this gene is shared by all species of Pneumocystis and has the least inter-species variation. The conserved part of the sequence is compared in NCBI as shared by multiple Pneumocystis species , and the homology is greater than 70%, meeting the genus-specific detection criteria. Its sequence is:

[0061] GTGTACGTTGCAAAGTACTCAGAAGAATTGTGGTAAGTAGTGAAATACAAATCGGGCTAGGATATAGCTGGTTTTCTGCGAAAATTGTTTTGGCAAATTG TTTATTCCTCTAAAAAATAGTAGGTATAGCACTGAATATCTCGAGGGAGTATGAAAATATTTATCTCAGATATTTAATCTCAAAATAACTATTTCTTAA A ATAAATAATCAGACTATGTGCGATAAGGTAGATAGTCGAAAGGGAAACAGCCCAGAACAGTAATTAAAGCTCCCCAATTAATATTAAGTGAAATAAAA

[0062] (2), the composition of PCR detection kit:

[0063] (1) DNA lysate:...

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Abstract

The invention provides a kit for the specific PCR (polymerase chain reaction) detection of pneumocystis, which comprises DNA lysate, a PCR reaction solution, positive control and negative control. The invention provides a detection method of the kit for the specific PCR detection of pneumocystis, which comprises the steps of sample pretreatment, sample DNA extraction, PCR amplification and PCR product observation. The kit for the PCR detection, disclosed by the invention has rigorous design; meanwhile, the detection method has the advantages of simpleness and rapidness in operation, high sensitivity, strong specificity and accuracy and objectivity in result determination.

Description

technical field [0001] The invention relates to a PCR detection kit and a detection method thereof, in particular to a PCR rapid detection kit and a detection method suitable for detecting Pneumocystis in humans, belonging to the technical field of biomedicine. Background technique [0002] Human Pneumocystis pneumonia (PCP) caused by Pneumocystis jiroveci (PJ) is more common in immunocompromised or deficient populations, and the mortality rate is extremely high. With the expansion of high-risk groups such as cancer chemotherapy patients, organ transplant recipients receiving immunosuppressant therapy, and autoimmune disease patients, especially since the first case of AIDS was discovered in 1984, the incidence has shown a sharp increase. PCP is the main complication and cause of death of AIDS patients, and is regarded as the "marker disease" of AIDS. [0003] The early diagnosis of PCP is difficult because: the clinical symptoms, signs, chest X-ray and routine laboratory e...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 孟维娜郭增柱郭敏敏
Owner 苏州深久医药生物技术有限公司
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