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Strain generating antioxidant

A technology of antioxidants and bacterial strains, applied in the direction of fungi, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as complex extraction process, loss of biodiversity and ecological environment, destruction of animal and plant resources, etc.

Inactive Publication Date: 2011-10-05
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the content of natural antioxidant substances in animals and plants is low, and the extraction process is complicated, and the extraction from these resources will inevitably cause serious damage to these animal and plant resources, resulting in loss of biodiversity and ecological environment

Method used

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Experimental program
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Effect test

specific Embodiment approach 1

[0013] Specific embodiment 1: The anti-oxidant-producing bacterial strain of this embodiment is Penicillium JTLR-1, which is preserved in the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee, with a preservation date of March 21, 2011, and a preservation number of CGMCC No. 4701.

[0014] Penicillium JTLR-1 was cultured in PDA solid medium or Chase solid medium, and there was white flocculent mycelium on the surface of the medium in 1 to 2 days, which grew fast and had a soft texture. After 2 days, there was a small amount of red substance in the center of the bottom of the plate Produced, the edge is light yellow, conidia can be produced after 5 to 7 days, the mycelium on the front of the colony is mixed with a large number of conidia, there is no exudate on the surface, the color of the conidia is dark green, and the back of the colony is red and yellow mixed. After Penicillium JTLR-1 was cultured on PDA liquid medium or C...

specific Embodiment approach 2

[0016] Specific embodiment two: this embodiment is obtained from the separation and purification of Corylus stem bark, and the specific steps are: 1. Rinse the hazel bark with part of the xylem to remove impurities with sterile water, and then use 75% of the mass concentration Soak in alcohol for 1 min, then rinse off the alcohol with sterile water, and cut into small pieces of hazel bark with an area of ​​0.5cm×0.5cm with sterile scissors; 2. Place the small pieces of hazel bark on the PDA agar solid medium plate 1. Cultivate at a constant temperature at 28°C until mycelium grows obviously around the small pieces of bark; 3. Inoculate the bacteria on PDA agar solid medium by picking the tip mycelia, and culture at a constant temperature of 28°C for 3 to 5 days , Pick a single colony, use the PDA agar solid medium plate streak culture method, and obtain Penicillium JTLR-1, which can produce antioxidants, after repeated isolation, screening, and purification.

[0017] Molecular...

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Abstract

The invention relates to a strain generating an antioxidant, in particular to a penicillium strain. The penicillium strain can generate the antioxidant and provide a microbial resource basis for the obtaining of the antioxidant. The strain generating the antioxidant is penicillium JTLR-1 and is preserved in the CGMCC (China General Microbiological Culture Collection Center) with a preserving date of March 21, 2011, and a preserving number of CGMCC No. 4701. The penicillium JTLR-1 can generate the antioxidant and provide the microbial resource basis for the obtaining of the antioxidant.

Description

technical field [0001] The present invention relates to a Penicillium strain. Background technique [0002] Free radicals are intermediate metabolites of various biochemical reactions in life activities. Excessive generation of free radicals or slow clearance will cause various damages to the body at the molecular level, cellular level, and tissue and organ level, accelerate the aging process of the body and induce various diseases. Free radicals attack life macromolecules and various organelles and cause tissue damage, which is the root cause of body aging and a major cause of malignant diseases such as tumors. [0003] Antioxidant substances (antioxidants) refer to a class of substances that can scavenge oxygen free radicals, inhibit or eliminate and slow down oxidation reactions. The development and research of antioxidants has been widely concerned by people. Natural antioxidants have been developed from simply being antioxidants in oils and fat-containing foods to bei...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P1/02C12R1/80
Inventor 金涛邹积宏
Owner HEILONGJIANG UNIV