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Method and device for producing dark spot on basis of transparent medium small ball

A technology of transparent medium and generation method, applied in microscopes, optics, instruments, etc., can solve the problems of unreachable focusing spot volume, inability to extinction effect, small excitation light range, etc., to increase optical power extinction effect and increase practicability , to achieve the effect of easy principle

Inactive Publication Date: 2011-10-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The reason is that limited by the diffraction limit, the volume of the focused spot cannot reach the required size
STED (stimulated emission depletion stimulated emission depletion) super-resolution microscopy method provides a better direction for the development of optical microscopes, but STED light needs to produce a hollow spot for extinction to make the range of excitation light smaller and realize true point detection
However, the existing technology relies on tangentially polarized light to focus through a microscope objective to obtain a hollow spot, but the spot size is still large, which cannot achieve a good extinction effect.

Method used

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  • Method and device for producing dark spot on basis of transparent medium small ball
  • Method and device for producing dark spot on basis of transparent medium small ball
  • Method and device for producing dark spot on basis of transparent medium small ball

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Embodiment Construction

[0033] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments, but the present invention is not limited thereto.

[0034] Such as figure 2 As shown, a device for generating dark spots based on transparent medium balls includes: a light source 1 , a microscope objective lens 4 , a cover glass 3 and transparent medium balls 2 . Wherein, the microscope objective lens 4, the transparent medium ball 2 and the cover glass 3 are located on the optical path of the polarized working light beam emitted by the light source 1 used. The transparent medium ball 2 is a micron-scale transparent medium ball with a size of 1-10 um and a refractive index of 1.4-2. The transparent medium ball 2 is located near the focal plane of the microscope objective lens 4, and is fixed by the cover glass 3, and an ultra-small focused dark spot will be obtained at the exit end of the transparent medium ball 2. It can be used to realize the exti...

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PUM

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Abstract

The invention discloses a method and a device for producing a dark spot on the basis of a transparent medium small ball. The method comprises the following step that: a tangent polarization light beam is incident to the microndimension transparent medium small ball, so that the dark spot is obtained at the emergent end of the microndimension transparent medium small ball. The device comprises a device for producing the tangent polarization light beam, a microscopic objective lens, the microndimension transparent medium small ball and cover glass for fixing the microndimension transparent medium small ball sequentially; and the tangent polarization light beam is initially focused by the microscopic objective lens and further focused by the microndimension transparent medium small ball so as to obtain the dark spot. In the invention, a single light beam serves as a working light beam of the system, so the system cost is saved and the practicability is enhanced; meanwhile, the size of the focused dark spot is up to 0.414 lambdas and much smaller than that of the dark spot focused by a common tangent polarization light beam. Furthermore, the quality of the light beam is improved, so actual application is facilitated.

Description

technical field [0001] The invention belongs to the field of optical metrology and super-resolution imaging, and in particular relates to a dark spot focusing method and device for realizing an ultra-small focusing field. Background technique [0002] With the development of current technology, the objects that come into contact with tend to be miniaturized, and the objects that need to be manufactured or observed are increasingly beyond the range that can be observed by traditional optical microscopes. The reason is that limited by the diffraction limit, the volume of the focused spot cannot reach the required size. STED (stimulated emission depletion stimulated emission depletion) super-resolution microscopy method provides a better direction for the development of optical microscopes, but STED light needs to produce a hollow spot for extinction to make the range of excitation light smaller and realize True point detection. However, the existing technology relies on focu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B27/58G02B21/24
Inventor 匡翠方库玉龙刘旭章海军张冬仙李海峰
Owner ZHEJIANG UNIV
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