Duck flavivirus and inactivated vaccine thereof

A duck yellow virus, inactivated vaccine technology, applied in the direction of antiviral agents, viruses/phages, virus antigen components, etc., to achieve the effects of convenient operation, simple process and high safety

Inactive Publication Date: 2011-10-19
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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  • Application Information

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Problems solved by technology

At present, there is no vaccine aga

Method used

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Embodiment 1

[0016] Duck flavin virus WR strain of the present invention is obtained in the following manner:

[0017] (1) Isolation and culture of the virus: In 2010, the ovarian tissue was aseptically collected from dead breeder ducks with abnormal egg production, homogenized according to conventional methods, repeatedly frozen and thawed 4 times, and centrifuged at 12 000 r / min at 4°C After 10 min, the supernatant was aspirated under aseptic conditions and frozen for later use.

[0018] (2) Isolation and culture of pathogenic poultry embryos: Inoculate five 10-day-old Muscovy duck embryos with the isolated virus through the allantoic cavity, 0.3 mL each, and incubate at 37°C for 10 days of continuous observation. The virus was continuously passed on for 5 generations until it became stable, and 100% of the Muscovy duck embryos could be killed, and the virus Muscovy duck embryo-adapted strain, namely duck yellow virus WR strain, was obtained.

[0019] Duck yellow virus inactivated vacci...

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Abstract

The invention provides a duck flavivirus and an inactivated vaccine thereof and belongs to the field of veterinarian lemology. The duck flavivirus is a WR strain of duck flavivirus. The WR strain is inoculated with a Muscovy duck embryo, inactivated by formaldehyde and then added with a white oil adjuvant for emulsification to prepare the inactivated vaccine. The invention proposes to prepare inactivated vaccine against duck flavivirus by use of the WR strain of duck flavivirus for the first time; and through the research and preparation of the vaccine, the defect that the disease cannot be effectively prevented at present in China is solved, and the loss caused by the sharp reduction of eggs laid by each duck due to the current high duck flavivirus morbidity can be effectively avoided. By using the WR strain of duck flavivirus to prepare the inactivated vaccine, the process is simple, and the operation is convenient; and the prepared vaccine has the advantages of good antigenicity and high safety, does not bring any bad influence on external environment, and passes the safety evaluation easily.

Description

technical field [0001] The invention relates to a duck yellow virus and an inactivated vaccine thereof, belonging to the field of veterinary epidemiology. Background technique [0002] Since the beginning of April 2010, breeding ducks and laying ducks in Jiangsu, Zhejiang, Shandong, Fujian and other provinces of my country have experienced a disease characterized by a rapid decline in feed intake, a rapid decline in egg production rate, and even shutdown and death to varying degrees. Commonly known as (egg) duck egg production drop, duck hemorrhagic ovariitis (duck hemorrhagic ovaritis, DHO). Various breeds of laying ducks, Cherry Valley ducks and Taiwan white-modified ducks have occurred, but it is mainly seen in laying ducks. Ducks with a high egg production rate will drop rapidly from the peak egg production or high egg production rate to 20% to 30% about 4 to 5 days after the disease, and the serious ones will stop production about 7 days after the onset; The egg produc...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/12A61P31/14C12R1/93
Inventor 黄瑜施少华万春和傅光华程龙飞陈红梅林芳林建生
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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