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Method for mutagenizing micro algae to generate docosahexaenoic acid by use of ion beam bioengineering

A technology for the yield of docosahexaenoic acid and docosahexaenoic acid, which is applied in the field of bioengineering, can solve the problems of unfavorable downstream separation and purification, complicated autotrophic culture equipment, and low concentration of active ingredients, and achieves Good economic and social benefits, wide application range, cheap fermentation raw materials

Inactive Publication Date: 2011-10-19
武汉中科光谷绿色生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The industry has used the autotrophy of microalgae to synthesize polyunsaturated fatty acids in photoculture reactors, but the autotrophic culture equipment is complicated and the output rate is low
Moreover, the concentration of active ingredients in the algae liquid is low, which is not conducive to downstream separation and purification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] A method for producing docosahexaenoic acid by using ion beam bioengineering to induce microalgae to produce docosahexaenoic acid, comprising the steps of:

[0012] 1) Chemical mutagenesis: choose Cryptidinosa as the starting algae species, cultivate it on a slope according to the conventional method, cultivate the inoculum bottle in a culture room at 25°C, and dilute the microalgae in the seed solution cultivated for about three days with artificial seawater solution, The dilution is controlled within 100 times. Then the diluted microalgae suspension obtained is mixed with the chemical mutagen solution nitrosoguanidine solution in a specific ratio. Below 25°C, treat for 20 minutes; then centrifuge in a centrifuge for 5 minutes, discard the supernatant, and then add artificial seawater.

[0013] Wherein, the concentration of the nitrosoguanidine solution used in the present invention is 0.1 g / L, and the volume ratio of the chemical mutagen to the microalgae suspension ...

Embodiment 2

[0017] A method for producing docosahexaenoic acid by using ion beam bioengineering to induce microalgae to produce docosahexaenoic acid, comprising the steps of:

[0018] 1) Chemical mutagenesis: choose Cryptidinosa as the starting algae species, cultivate it on a slope according to the conventional method, cultivate the inoculum bottle in a culture room at 25°C, and dilute the microalgae in the seed solution cultivated for about three days with artificial seawater solution, The dilution is controlled within 100 times. Then the diluted microalgae suspension obtained is mixed with the chemical mutagen solution nitrosoguanidine solution in a specific ratio. Below 27°C, treat for 25 minutes; then centrifuge in a centrifuge for 5 minutes, discard the supernatant, and then add artificial seawater.

[0019] Wherein, the concentration of the nitrosoguanidine solution used in the present invention is 0.2 g / L, and the volume ratio of the chemical mutagen to the microalgae suspension ...

Embodiment 3

[0023] A method for producing docosahexaenoic acid by using ion beam bioengineering to induce microalgae to produce docosahexaenoic acid, comprising the steps of:

[0024] 1) Chemical mutagenesis: choose Cryptidinosa as the starting algae species, cultivate it on a slope according to the conventional method, cultivate the inoculum bottle in a culture room at 25°C, and dilute the microalgae in the seed solution cultivated for about three days with artificial seawater solution, The dilution is controlled within 100 times. Then the diluted microalgae suspension obtained is mixed with the chemical mutagen solution nitrosoguanidine solution in a specific ratio. Below 30°C, treat for 30 minutes; then centrifuge in a centrifuge for 5 minutes, discard the supernatant, and then add artificial seawater.

[0025] Wherein, the concentration of the nitrosoguanidine solution used in the present invention is 0.3 g / L, and the volume ratio of the chemical mutagen to the microalgae suspension ...

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Abstract

The invention discloses a method for mutagenizing micro algae to generate docosahexaenoic acid by use of ion beam bioengineering. The method comprises the following steps: 1) chemical mutagenesis: selecting crypthecodinium cohnii as the starting algae specie, performing slant culture according to a conventional method, culturing a seed inoculation bottle in a culture room at 25 DEG C, and diluting the micro algae in the seed liquid after about three days of culture with an artificial sea water solution; 2) ion beam mutagenesis: uniformly spreading the processed algae liquid in a sterile empty culture vessel, adding 20-30% glycerol for protection, drying with sterile air, placing a flat plate coated with micro algae into an ion implanter target room, and performing ion implantation; and 3) after the ion implantation is finished, screening unicellular algae by utilizing a solid medium, and picking the high-yield algae seeds from the shake flask in which the yield of docosahexaenoic acid cultured in the shake flask is 10%-20% higher than that of a control group. The method disclosed by the invention has the advantages of simple breeding method, relatively low cost and short breeding period.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for producing polyunsaturated fatty acids by microalgae fermentation. Background technique [0002] Docosahexaenoic acid (DHA) is a kind of polyunsaturated fatty acids (PUFAs), which is an important component of phospholipids in animal cell membranes and determines the fluidity and deformability of cell membranes. It plays an important role in the prevention and treatment of various diseases and certain cancers, and the intake of sufficient PUFAs is crucial to the development of vision and nerves of fetuses and young children. European and American countries now have a wide range of applications for DHA, mainly in food and health care medicines. Applications in food include infant milk powder, milk, cheese, edible vegetable oil, baking oil, etc. Consumers have a relatively high degree of awareness and acceptance of DHA. DHA formula milk powder has been listed in...

Claims

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Application Information

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IPC IPC(8): C12N13/00C12N15/01C12P7/64C12R1/89
Inventor 姚黎明
Owner 武汉中科光谷绿色生物技术有限公司
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