Application of Chinese gall extract for controlling streptococcal disease in aquaculture animals
A gallnut, extract technology, applied in the direction of application, antibacterial medicine, animal feed, etc.
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Embodiment 1
[0013] Embodiment 1: the preparation of Galla gall extract
[0014] Gallnuts harvested in the same year were taken, crushed to 100 mesh, extracted three times with 90% ethanol, the amount of solvent was 5 times, 3 times, and 3 times the weight of the medicinal material, and the extraction time was 3 hours, 3 hours, and 3 hours respectively. Combine the extracts, concentrate, reclaim 90% ethanol (recovered ethanol accounts for 90% of the amount of ethanol added), then filter, and the filtrate continues to be concentrated to no solvent to obtain a light brown extract, which is Galla galla extract.
Embodiment 2
[0015] Embodiment 2: the preparation of Galla gall extract
[0016] Gallnuts harvested in the same year were taken, crushed to 160 mesh, and extracted twice with 70% ethanol. The amount of solvent was 5 times and 4 times the weight of the medicinal material respectively, and the extraction time was 3 hours and 2 hours respectively. Combine the extracts, concentrate, reclaim 80% ethanol (recovered ethanol accounts for 80% of the amount of ethanol added), then filter, and the filtrate continues to be concentrated to no solvent to obtain a light brown extract, which is Galla galla extract.
Embodiment 3
[0017] Embodiment 3: the preparation of Galla gall extract
[0018] Gallnuts harvested in the same year were taken, crushed to 120 mesh, extracted three times with 60% ethanol, the amount of solvent was 4 times, 4 times, and 3 times the weight of the medicinal material, and the extraction time was 3 hours, 2 hours, and 2 hours respectively. Combine the extracts, concentrate, reclaim 85% ethanol (recovered ethanol accounts for 85% of the amount of ethanol added), then filter, and the filtrate continues to be concentrated to no solvent to obtain a light brown extract, which is Galla galla extract.
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