Preparation method of composite extract for fermentation type tobacco
A fermentation type and extract technology is applied in the field of preparation of fermented tobacco compound extract, which can solve problems such as application limitations, and achieve the effects of increasing sweetness, reducing irritation and improving taste.
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Embodiment 1
[0036] 1) Culture medium preparation
[0037] a. Raw material pre-treatment: Fructus Rosa Rosa, sweet and sour horn are crushed into large granules, Rosa rosa, Lycium barbarum, and sweet and sour horn are mixed by weight respectively in 20 parts, 20 parts, and 60 parts, and the raw material after mixing is mixed with water Reflux extraction for 3 hours according to the mass ratio of 1:8, filter with a 0.8 μm microporous membrane, concentrate, and concentrate to a relative density of 1.07 to obtain a composite extract;
[0038] b. Culture medium preparation: add 0.5% glucose and 0.5% sodium chloride of the compound extract mass to the prepared compound extract to prepare the medium;
[0039] 2) Vaccination
[0040] Sterilize the medium at 121°C for 20 minutes, and inoculate the aroma-producing active dry yeast into the medium under aseptic conditions, and the inoculation ratio is 0.5% of the mass of the medium;
[0041] 3) fermentation
[0042] Ferment for 3 days at a pH val...
Embodiment 2
[0046] 1) Culture medium preparation
[0047] a. Raw material pre-treatment: Fructus Rosa Rosa, sweet and sour horn are broken into big granular shape, and Rosa rosae, medlar, sweet and sour horn are mixed by weight respectively 10 parts, 15 parts, 50 parts, the raw material after mixing and water Reflux extraction for 2 hours according to the mass ratio of 1:5, filter with a 0.8 μm microporous membrane, concentrate, and concentrate to a relative density of 1.03 to obtain a composite extract;
[0048] b. Culture medium preparation: add 0.1% glucose and 0.1% sodium chloride of the compound extract mass to the prepared compound extract to prepare the medium;
[0049] 2) Vaccination
[0050] Sterilize the medium at 121°C for 30 minutes, and inoculate the aroma-producing active dry yeast into the medium under aseptic conditions, and the inoculation ratio is 0.01% of the mass of the medium;
[0051] 3) fermentation
[0052] Ferment for 9 days at a pH value of 2.5, a stirring speed...
Embodiment 3
[0056] 1) Culture medium preparation
[0057] a. Raw material pre-treatment: Fructus Rosa Rosa, sweet and sour horn are broken into large granular shape, and Rosa rosae, medlar, sweet and sour horn are mixed by weight respectively 25 parts, 25 parts, 75 parts, the raw material after mixing and water Reflux extraction for 5 hours according to the mass ratio of 1:15, filter with a 0.8 μm microporous membrane, concentrate, and concentrate to a relative density of 1.12 to obtain a composite extract;
[0058] b. Culture medium preparation: add 5% glucose and 0.9% sodium chloride of the compound extract quality to the prepared compound extract to prepare the culture medium;
[0059] 2) Vaccination
[0060] Sterilize the medium at 121°C for 15 minutes, and inoculate the aroma-producing active dry yeast into the medium under aseptic conditions, and the inoculation ratio is 2% of the mass of the medium;
[0061] 3) fermentation
[0062] Ferment for 2 days at a pH value of 7.0, a sti...
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