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Preparation method and purpose of copper-loaded chitosan antiseptic

A technology of copper-loaded chitosan and antibacterial agent, which is applied in the field of preparation of copper-loaded chitosan antibacterial agent, can solve the problems of expensive products, achieve the effects of protecting intestinal mucosa, strong antibacterial properties, and inhibiting the proliferation of pathogenic bacteria

Inactive Publication Date: 2011-11-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Ag + Best, it has a strong killing effect on various pathogenic bacteria, and the required concentration is extremely low, but the product is expensive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, a kind of preparation method of copper-loaded chitosan antibacterial agent, carries out following steps successively:

[0025] 1), at 25 DEG C, in the hydrochloric acid solution of mass concentration 0.1%, add the chitosan that molecular weight is 2kDa, obtain chitosan acid solution; The mass concentration of chitosan in chitosan acid solution is 1%;

[0026] 2), utilize the hydrochloric acid solution of mass concentration 0.1% to regulate chitosan acid solution, make its pH value be 3.5; Thus form microparticle emulsion;

[0027] 3), centrifuge (4000rpm / minute speed, time is 15 minutes) to separate the microparticle emulsion to obtain microparticles;

[0028] Slowly adding the microparticles into the cupric chloride solution with a copper ion concentration of 500 mg / L under agitation to obtain a mixed solution, the cupric chloride and chitosan in step 1) have a weight ratio of 15%; then use the concentration 0.5mol / L sodium hydroxide solution to adjust t...

Embodiment 2

[0042] Embodiment 2, a kind of preparation method of copper-loaded chitosan antibacterial agent, carries out following steps successively:

[0043] 1), at 40 DEG C, in the hydrochloric acid solution of mass concentration 1%, add the chitosan that molecular weight is 10kDa, obtain chitosan acid solution; The mass concentration of chitosan in chitosan acid solution is 3%;

[0044] 2), utilize the hydrochloric acid solution of mass concentration 1% to regulate chitosan acid solution, make its pH value be 4.5; Thus form microparticle emulsion;

[0045] 3), centrifugally separate the microparticle emulsion to obtain microparticles;

[0046] Slowly adding the microparticles into the copper nitrate solution with a copper ion concentration of 800mg / L under stirring conditions, to obtain a mixed solution, the weight ratio of copper nitrate to chitosan in step 1) is 25%; then the utilization concentration is 1mol / L Potassium hydroxide solution adjusted the pH value of the mixed solutio...

Embodiment 3

[0059] Embodiment 3, a kind of preparation method of copper-loaded chitosan antibacterial agent, carries out following steps successively:

[0060] 1), at 50 DEG C, in the acetic acid solution of mass concentration 2%, add the chitosan that molecular weight is 80kDa, obtain chitosan acid solution; The mass concentration of chitosan in chitosan acid solution is 5%;

[0061] 2), utilize the acetic acid solution of mass concentration 2% to regulate chitosan acid solution, make its pH value be 5; Thus form microparticle emulsion;

[0062] 3), centrifugally separate the microparticle emulsion to obtain microparticles;

[0063] Slowly adding the microparticles into the copper sulfate solution with a copper ion concentration of 1000 mg / L under stirring conditions to obtain a mixed solution, the weight ratio of copper sulfate to chitosan in step 1) is 30%; then the utilization concentration is 2mol / L The sodium bicarbonate solution adjusted the pH value of the mixed solution to 6, an...

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Abstract

The invention discloses a preparation method of a copper-loaded chitosan antiseptic, comprising the following steps: 1) adding chitosan into an acid solution with a mass concentration of 0.1 to 5% so as to obtain a chitosan acid solution; 2) adjusting the pH value of the chitosan acid solution to be 3 to 5, thereby forming corpuscule emulsion; 3) centrifuging the corpuscule emulsion to obtain corpuscules, adding corpuscules into a copper salt solution, adjusting the pH value of the solution to be 4.5 to 5.5, and standing the mixture for reaction at room temperature for 5 to 10 hours so as to obtain emulsion; 4) adjusting the pH value of the emulsion to be 7 to 9, carrying out rinsing 3 to 7 times, carrying out refiltering or centrifuge dewatering, and collecting solid products; 5) crushing the solid products after drying so as to obtain the copper-loaded chitosan antiseptic. The purpose of the copper-loaded chitosan antiseptic is to use the antiseptic as a feed additive for aquatic livestock.

Description

technical field [0001] The invention relates to a preparation method and application of a copper-loaded chitosan antibacterial agent. Background technique [0002] Various pathogenic bacteria exist in the digestive tract of animals, and these pathogenic bacteria can produce toxins, directly or indirectly destroy the shape and function of the intestinal mucosa, thereby reducing the feed efficiency and growth performance of livestock and poultry. If the number of intestinal pathogenic bacteria exceeds a certain value, indigestion and diarrhea may occur in mild cases, and enterogenous systemic infection may occur in severe cases, even life-threatening, which makes antibiotics widely used in aquaculture production. The extensive use of antibiotics has played a role in promoting animal growth, disease prevention and disease resistance to a certain extent. However, long-term use and abuse of antibiotics will cause adverse consequences such as drug resistance, drug-resistant gene ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/34A61K47/36A23K1/16A61P31/04A23K20/195
Inventor 王敏奇谢小利王超杜勇杰陶文静许梓荣
Owner ZHEJIANG UNIV
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