Feedback-type pneumatic-control pressure stress cell culture device

Abstract

The invention relates to a feedback-type pneumatic-control pressure stress cell culture device and is characterized in that a high-pressure gas storage tank is connected with a three-way pipe (a) through a switch, a pressure relief valve and a connecting pipe; the three-way pipe (a) is communicated with a lower connecting pipe and the connecting pipe respectively; a syringe is connected with a micro pressure pump; the connecting pipe is connected with a three-way pipe (b); the three-way pipe (b) is connected with a pipe, a pressure meter and the upper end of an aseptic filtration device respectively; the lower end of the aseptic filtration device is communicated with a needle; a rubber stopper is arranged on a measuring cylinder type glass container; an upper outer reinforcement plate is arranged on the rubber stopper; a lower outer reinforcement plate is arranged below the measuring cylinder type glass container; the needle passes through the rubber stopper and enters the inside of the measuring cylinder type glass container; and a cell culture dish is arranged at the bottom of the measuring cylinder type glass container. The invention solves the problems that a closed container is difficult to open or close and can not tolerate a higher pressure stress. With the smart combination of the micro pressure pump and the pressure stress cell culture device, the pressure stress cellculture device has a simple structure, is convenient to operate, has a reliable effect and is applied to biological scientific research institutions.

Description

technical field [0001] The invention relates to biological scientific research equipment, in particular to a feedback air-controlled pressure stress cell culture device. Background technique [0002] Before the invention, the compressive stress cell culture devices used in biological research are relatively expensive, and the control of continuous compressive stress is not very ideal. The effect of mechanical stress on cell growth has also been a persistent difficulty. Therefore, many laboratories are unable to carry out this research, or the cost of purchasing one is too high, or the continuous compressive stress is not ideal, resulting in inaccurate effects and affecting experimental data. [0003] For airtight containers that are both easy to open and close and can withstand a certain amount of compressive stress (especially positive pressure), the current effects are not ideal. Present device has air inlet and air outlet more, and both are installed valves, have increa...

AI Technical Summary

Problems solved by technology

Therefore, many laboratories are unable to carry out this research, or the cost of purchasing one is too high, or the continuous compressive stress is not ideal, resulting in inaccurate results and affecting experimental data

[0003] For airtight containers that are easy to open and close and can withstand a certain amount of compressive stress (especially positive pressure), the current effects are not satisfactory.

Most of the current devices have an air inlet and an air outlet, and both of them are equipped with valves, which increases the complexity of the device

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