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Detection method for gamma-aminobutyric acid content in food by using high performance liquid chromatography method

A high-performance liquid chromatography and aminobutyric acid technology, which is applied in the field of high-performance liquid chromatography for detecting the content of γ-aminobutyric acid in food, can solve the problem of not formulating γ-aminobutyric acid and the like, and achieve reliable methods, The effect of reliable implementation and easy implementation

Inactive Publication Date: 2011-11-02
宁波谱尼测试技术有限公司
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  • Application Information

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Problems solved by technology

In 2009, the Ministry of Health of my country approved γ-aminobutyric acid as a new resource food, but there is no standard detection method for the content of γ-aminobutyric acid in food

Method used

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  • Detection method for gamma-aminobutyric acid content in food by using high performance liquid chromatography method
  • Detection method for gamma-aminobutyric acid content in food by using high performance liquid chromatography method
  • Detection method for gamma-aminobutyric acid content in food by using high performance liquid chromatography method

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Experimental program
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Effect test

Embodiment 1

[0033] Example 1, detection of precision and recovery rate of gamma-aminobutyric acid content in food:

[0034] Prepare 0.4mol / L borate buffer solution (pH=10.2): Weigh 3.43g borax and 0.25g boric acid, add 80mL water to dissolve, adjust pH=10.20 with 5mol / L sodium hydroxide solution, and set volume to 100mL.

[0035] Preparation of derivative solution: Weigh 0.1g of o-phthalaldehyde, add 30μL of 2-mercaptoethanol, add 4.0mL of methanol, fully dissolve, and dilute to 5.0mL with borate buffer solution.

[0036] Weigh 2.0021g (accurate to 0.0001g) of the well-mixed sample to be tested, add pure water, make up to 10mL, vortex and mix thoroughly, ultrasonically extract for 2min, centrifuge at 3000r / min for 5.0min, pass through a 0.45μm filter membrane After filtration, it was derivatized and analyzed online by high performance liquid chromatography.

[0037] Weigh 100.00mg of γ-aminobutyric acid standard substance, dissolve it with secondary water and dilute it to a 100mL volumet...

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Abstract

The invention relates to a detection method for gamma-aminobutyric acid (GABA) content in food by using high performance liquid chromatography (HPLC) method. The method is characterized by: carrying out sufficient whirling through double distilled water, and an ultrasonic extraction for a sample requiring detection, then carrying out a centrifugating to obtain supernatant, followed by filtering the supernatant to obtain the sample liquid; weighing the GABA standard substance, then dissolving the GABA through the double distilled water, and carrying out a metered volume for the GABA solution to adopt the solution as a standard stock solution, further dissolving the standard stock solution through the double distilled water to obtain a standard working solution having a concentration gradient; adopting o-phthalaldehyde and 2-mercaptoethanol as derivating agents, and carrying out a derivation for the sample liquid through a HPLC equiped with a online derivation apparatus, followed by carrying out a quantitative detection through a external standard method. The method for detecting the GABA content in the food is quick and effective, and has a linearity range of 0.5-1000 mg / L, a recovery rate more than 88.2%, a relatibe standard deviation (RSD) less than 5%. In addition, with the present invention, a reliable and convenient method is provided for detecting the GABA content in the food,; requirements of researches and productions are met.

Description

technical field [0001] The present invention relates to a method for measuring the content of gamma-aminobutyric acid in food by high-performance liquid chromatography, in particular to a method for measuring the content of gamma-aminobutyric acid in food by a high-performance liquid chromatography equipped with an online derivatizer method of detection. Background technique [0002] γ-aminobutyric acid (γ-aminobutyric acid, GABA), also known as aminobutyric acid, pipecolic acid, is a non-protein amino acid widely distributed in animals and plants. Seeds, rhizomes and tissue fluids of plants such as beans, ginseng, and Chinese herbal medicines all contain GABA. GABA is an important inhibitory neurotransmitter that has been studied deeply in medicine at present. It participates in various metabolic activities and has high physiological activity. In 2009, the Ministry of Health of my country approved γ-aminobutyric acid as a new resource food, but there is no standard detect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/36
Inventor 宋薇
Owner 宁波谱尼测试技术有限公司
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