Method for quickly identifying resistance of Chinese cabbage germplasm to black-spot disease

An identification method, Chinese cabbage technology, applied in horticultural methods, botanical equipment and methods, gardening, etc., can solve problems such as labor-intensive, unguaranteed, and inaccurate identification results, so as to avoid unsuitable climate conditions and save manpower and material resources , The effect of short identification cycle

Inactive Publication Date: 2011-11-16
INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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Problems solved by technology

The inoculation method adopts the method of dripping spore suspension, which has the following disadvantages: manual drip inoculation of each strain is

Method used

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  • Method for quickly identifying resistance of Chinese cabbage germplasm to black-spot disease
  • Method for quickly identifying resistance of Chinese cabbage germplasm to black-spot disease
  • Method for quickly identifying resistance of Chinese cabbage germplasm to black-spot disease

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Experimental program
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Embodiment Construction

[0020] 1.1 Test materials

[0021] The tested black spot fungus was Alternaria brassicae, and the carbon source halved PDA medium was added to filter-sterilized cabbage juice for sporulation before inoculation; the tested Chinese cabbage seeds were sown In flowerpots (8cm*8cm) equipped with sterilized substrates, leave 1-3 plants in each pot after emergence;

[0022] 1.2 Inoculation method

[0023] Spray inoculation with spore suspension, put the test plants into plastic boxes after inoculation, and keep them moist for 24 hours with plastic wrap. Set 3 repetitions for each test material, 30 seedlings for each repetition, with clear water as the control, set 1.0×10 3 , 1.0×10 4 , 1.0×10 5 , 1.0×10 6 Pfu / mL four inoculation concentrations of spore suspension and three seedling ages of 3-4, 5-6, 7-8 leaf stages were induced at 25-30°C, placed in a light culture room, and the first day after inoculation 7d, 10d and 14d investigation condition index.

[0024] 1.3 Classificat...

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Abstract

The invention provides a method for quickly identifying the resistance of Chinese cabbage germplasm to a black-spot disease, which is characterized by comprising the following sequential steps of: firstly, preparing a material to be tested; secondly, inoculating; and thirdly, grading a disease index, wherein appropriate concentration of black-spot disease suspending liquid and the seedling age for screening disease-resistant Chinese cabbage are determined; and compared with the inoculation during the adult-plant growth period in field, the method avoids influences of the factors of unsuitability of climatic conditions, plant diseases and insect pests in the environment, and the like, is easy to control the conditions for indoor disease resistance screening during the seedling period, and has short cycle of identification and high speed; and a great amount of manpower and material resources can be saved.

Description

technical field [0001] The invention belongs to the field of disease-resistant breeding of horticultural crops, and in particular relates to a rapid identification method for germplasm resistance to black spot of Chinese cabbage. Background technique [0002] Black spot of Chinese cabbage is caused by fungi of the genus Alternaria, including Alternaria brassicae, A. brassicicola and A. japonica. Black spot of Chinese cabbage is a kind of disease that often occurs, and it is widely distributed, and it has occurred in my country and the world. As early as the 1940s, the distribution of cabbage black spot was relatively common in my country, but it had little effect on growth. In the late 1970s, the occurrence of the disease became more and more serious in my country, and it has become one of the main diseases of Chinese cabbage. It can cause a 20% to 40% reduction in production, and Wuhan is the area most affected by it. However, the effects of ecological control and chemical...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G7/06
Inventor 甘彩霞梅时勇崔磊何云启黄来春王晴芳丁自立张兴中梅雪飞高翔
Owner INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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