Digital automatic production method for umbilical cord mesenchymal stem cells

Abstract

The invention relates to a digital automatic production method for umbilical cord mesenchymal stem cells. The method is implemented by taking a culture flask as a cell growth carrier and combining a mechanical assembly line with a digital full-automatic bioreactor. The culture flask comprises three culture flasks which are arranged on the mechanical assembly line and are externally connected withthe bioreactor; the mechanical assembly line comprises a mechanical transmission device and an electronic control device; the electronic control device is used for controlling the mechanical transmission device to drive the culture flasks to complete actions such as oscillation, overturning, inclination by 90 degrees and the like in the assembly line according to processing data of the bioreactor; the bioreactor comprises a control system, a detection device, a gas supply device, a liquid filling device and a liquid removing device, and is used for analyzing and processing key parameters of the cell culturing process monitored by using the detection device and automatically completing gas supplying, liquid filling, liquid removing and collecting operation in cooperation with the mechanical assembly line, so that the entire cell culturing process is realized; and by adopting an umbilical cord explant, the umbilical cord mesenchymal stem cells can be amplified to 109 in an automatic production system.

Description

technical field [0001] The invention belongs to the field of cell culture and cell engineering, and relates to a digital automatic production method of umbilical cord mesenchymal stem cells. Background technique [0002] Traditional cell culture is mostly manual operation, which has many restrictions on various aspects of production, such as personnel quality, clean room level, operation management rules and regulations, etc. Despite the strict production management and perfect quality control, the influence of many human factors on the production of stem cells is inevitable, and the quality and stability of cells cannot be guaranteed. At present, bioreactors have been widely developed in the field of cell-scale production such as vaccine production at home and abroad. It can effectively improve the production efficiency of cells and related products and reduce the risk of cell production. However, the application of bioreactors to mesenchymal stem cells has not yet mat...

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problems of time-consuming and labor-intensive artificial culture of cells, poor stability of cell culture, high cost and low efficiency in the prior art, the present invention provides a digital automatic production method of umbilical cord mesenchymal stem cells, which is applied to the automatic and assembly line culture of cells, Reduce the influence factors of manual operation, ensure the quality and stability of cell culture, save the cost of cell culture, and improve the efficiency of cell culture

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