Digitalized automatic production system for mesenchymal stem cells of umbilical cord

Abstract

The invention relates to a digitalized automatic production system for mesenchymal stem cells of the umbilical cord, which is built by adopting three culture flasks as cell growth carriers, a combined mechanical production line and a digitalized full-automatic bioreactor. The three culture flasks are arranged on the production line and are externally connected with the bioreactor; the mechanical production line comprises a mechanical driving device and an electronic control device; the electronic control device is used for controlling the mechanical driving device according to processing dataof the bioreactor to ensure that the culture flasks finish oscillation, overturn, inclination at 90 degrees and other actions in the mechanical production line; the bioreactor comprises a control system, a detection device, a gas supply device, a liquid feeding device and a liquid draining device; the detection device is used for monitoring key parameters in the cell culture process; the key parameters are analyzed and processed by the bioreactor and are coordinated with the mechanical production line to automatically finish air supply, liquid feeding, liquid draining, collection operations, thereby the full process of cell culture is realized; and umbilical cord tissue blocks can be used for amplifying the mesenchymal stem cells of the umbilical cord to 109 in the automatic production system.

Description

technical field [0001] The invention belongs to the field of cell culture and cell engineering, and relates to a digital automatic production system of umbilical cord mesenchymal stem cells. Background technique [0002] Traditional cell culture is mostly manual operation, which has many restrictions on various aspects of production, such as personnel quality, clean room level, operation management rules and regulations, etc. Despite the strict production management and perfect quality control, the influence of many human factors on the production of stem cells is inevitable, and the quality and stability of cells cannot be guaranteed. At present, bioreactors have been widely developed in the field of cell-scale production such as vaccine production at home and abroad. It can effectively improve the production efficiency of cells and related products and reduce the risk of cell production. However, the application of bioreactors to mesenchymal stem cells has not yet mat...

AI Technical Summary

Problems solved by technology

[0003] In order to solve the shortcomings of the prior art, such as time-consuming and labor-intensive artificial culture of cells, poor stability of cell culture, high cost, and low efficiency, the present invention provides a digital automatic production system for umbilical cord mesenchymal stem cells, which is applied to cell automation and assembly lines Cultivation reduces the influence factors of manual operation, ensures the quality and stability of cell culture, saves the cost of cell culture, and improves the efficiency of cell culture

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