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Mycoplasma hyopneumoniae and Mycoplasma hyorhinosus dual inactivated vaccine and preparation method thereof

A technology of Mycoplasma hyopneumoniae and double inactivated vaccine, which is applied in the directions of bacterial antigen components and antibacterial drugs, can solve the problems of weak specificity and easy mutation of Mycoplasma hyopneumoniae, achieve good immunity and prevent multiple plasma Occurrence of meningitis or arthritis, effect of vaccine cost saving

Active Publication Date: 2011-11-30
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the inactivated Mycoplasma hyopneumoniae vaccine only has the monovalent vaccine imported from abroad, and Mycoplasma hyopneumoniae is prone to mutation, so it is not specific for diseases caused by Mycoplasma hyopneumoniae in domestic pig farms; while the Mycoplasma hyopneumoniae inactivated vaccine is available in China Foreign reports are blank

Method used

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  • Mycoplasma hyopneumoniae and Mycoplasma hyorhinosus dual inactivated vaccine and preparation method thereof
  • Mycoplasma hyopneumoniae and Mycoplasma hyorhinosus dual inactivated vaccine and preparation method thereof
  • Mycoplasma hyopneumoniae and Mycoplasma hyorhinosus dual inactivated vaccine and preparation method thereof

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Comparison scheme
Effect test

Embodiment 1

[0025] The preparation method of the dual inactivated vaccine of embodiment 1, mycoplasma swine pneumonia, mycoplasma hyorhinosum

[0026] The strain Mycoplasma suis pneumonia was selected as MR48 strain, which was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on September 19, 2006. The English name is Mycoplasma hyopneumoniae, and the address of the preservation unit is: Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing. Mycoplasma hyorhinosum CVCC361 strain was purchased from China Veterinary Drug Control Institute.

[0027] The steps to prepare the dual vaccine are as follows:

[0028] 1). Bacteria culture

[0029] 1.1 Culture of swine mycoplasma pneumoniae

[0030] The selected strain of Mycoplasma suis pneumonia was MR48 strain, which was preserved in the General Microorganism Center of China Committee for the Collection of Microbial Cultures on Sep...

Embodiment 2

[0097] Embodiment 2 Mycoplasma hyopneumoniae, Mycoplasma hyopneumoniae dual inactivated vaccine and the immune effect comparison of using Mycoplasma hyopneumoniae inactivated vaccine alone

[0098] 1. Materials The trial product of Mycoplasma hyopneumoniae and Mycoplasma hyorhina dual inactivated vaccine in Example 1; American Schering-Plough inactivated vaccine against Mycoplasma suis pneumonia (J strain) (batch number 100806).

[0099] 2 Animal experiment design

[0100] Select 60 weaned piglets aged 21 to 25 days, and divide them into 6 groups, 10 pigs in each group (see the table below); each pig in the 1st and 2nd groups was injected intramuscularly with the double inactivated vaccine of Mycoplasma hyopneumoniae and Mycoplasma hyorhina respectively in the neck 2ml; each pig in the third group was intramuscularly injected with 2ml of the commercialized vaccine of Mycoplasma hyopneumoniae in the neck; the fourth and fifth groups were used as the challenge control, without v...

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Abstract

The invention provides a combined inactivated vaccine against mycoplasma hyopneumoniae (MHP) and mycoplasma hyorhinis. The combined inactivated vaccine contains the MHP and the mycoplasma hyorhinis with preferred content as well as a Carbomer adjuvant with concentration of 10% (V / V). The invention further provides a preparation method of the combined inactivated vaccine against the MHP and the mycoplasma hyorhinis. The preparation method comprises the following steps: preparing production strains; preparing bacterium solution for producing seedlings; and inactivating, concentrating and blending to obtain the vaccine. The combined inactivated vaccine has the advantages of strong specificity and good immunity, thus solving the problem of specific infection caused by the MHP in the current domestic breeding farm and obtaining the mycoplasma hyorhinis vaccine under a blank state at home and abroad at present. The combined inactivated vaccine has the beneficial effects that the step of vaccine inoculation is simplified, trouble caused by a plurality of inoculation and easily produced side effects are avoided, and vaccine cost is saved, thus being especially applicable to preventing andtreating mixed infection diseases in the breeding farms at home and abroad and the like; and compared with the existing single vaccine, application range is widened and immune effect is enhanced.

Description

technical field [0001] The invention relates to a dual inactivated vaccine of Mycoplasma hyopneumoniae and Mycoplasma hyorhinosum and a preparation method thereof, belonging to the field of veterinary vaccines. Background technique [0002] Swine panting disease, also known as swine mycoplasma pneumonia or porcine endemic pneumonia, is a contact chronic respiratory infectious disease caused by Mycoplasma hyopneumoniae, which is prevalent all over the world. The diseased pigs mainly showed cough and wheezing, growth retardation, low feed conversion rate, and normal body temperature. During anatomy, the main lesions were in the lungs, especially in the heart lobe, middle lobe, and apical lobe of the two lungs, which were characterized by pancreatoid changes and fleshy changes. High morbidity and low mortality. Studies in recent years have found that Mycoplasma hyopneumoniae is mixed with porcine reproductive and respiratory syndrome virus and other pathogens, which further i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/116A61K39/02A61P31/04
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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