A method for rapid detection of goat six6 gene single nucleotide polymorphism and its application

Abstract

The invention discloses a method for rapidly detecting the single nucleotide polymorphism (SNP) of goat homeobox 6 (six6) gene, using the whole genome DNA of the goat to be tested containing the Six6 gene as a template, and using a primer pair P as Primers, PCR amplified goat Six6 gene; after the PCR product was digested with restriction endonuclease PstI, agarose gel electrophoresis was carried out; the genotype at position 232 of the goat Six6 gene was identified according to the electrophoresis results (with sequence Accession No.NM_001104993. 1 for reference); in animal production practice, since the Six6 gene single nucleotide variation or SNP has important effects on the formation of embryos, regulation of growth hormone and promotion of pituitary hormone secretion, the different genotypes of the site are related to growth and development Association analysis of trait data. The results showed that the tube circumference of CT genotype individuals was significantly larger than that of CC genotype individuals (P=0.047), indicating that CT genotype can be used as a molecular genetic marker to increase tube circumference in goats. Therefore, the detection method for rapid detection of Six6 gene SNP provided by the present invention is conducive to the rapid establishment of goat populations with excellent genetic resources in the marker-assisted selection (MAS) breeding of Chinese goat growth traits.

Description

technical field [0001] The invention belongs to the field of animal molecular breeding, and relates to the detection of gene single nucleotide polymorphism (SNP), in particular to a method for detecting the 232nd position of goat homeobox Six6 gene (with reference to sequence Accession No.NM_001104993.1) A single nucleotide variation or SNP method and its use in marker assisted selection (MAS) breeding for animal growth traits to rapidly establish a goat population with superior genetic resources. Background technique [0002] Goat is one of the important livestock species resources of human beings, and it plays an important role in the social economy and people's life of various countries. With the development of our country's economy and the improvement of people's living standards, the society's demand for goat products is constantly increasing. It is expected that goat breeding experts will cultivate fine varieties that can obtain goat products earlier, better and faster...

AI Technical Summary

Problems solved by technology

[0008] At present, the research on the SNP of the Six6 gene is mostly found in the in vitro expression analysis of organisms such as humans, mice, and marine fish, and is often used as a candidate gene related to organ dysfunction and important disease prediction, but is rarely seen in the SNP analysis of livestock and poultry. There are no related reports in goats

Images