A method for continuously extracting hypoglycemic active ingredients and estrogen active ingredients from Selaginella

An active ingredient and hypoglycemic technology, applied to endocrine system diseases, medical raw materials derived from ferns/filamentous plants, metabolic diseases, etc., can solve the problems of high cost of target products, loss of active ingredients, large amount of solvents, etc. Achieve the effect of avoiding a large number of crossover and loss, low production cost and significant effect

Active Publication Date: 2011-12-07
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, domestic enterprises or scientific research institutes mostly extract the active ingredients of Selaginella selaginella for a single product. Although this method can maximize the yield of the target ingredient, it causes the other active ingredients in Selaginella to be lost in vain. In addition, the amount of solvent used in the extraction process Large and labor-intensive, resulting in waste of resources and high cost of target products

Method used

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  • A method for continuously extracting hypoglycemic active ingredients and estrogen active ingredients from Selaginella
  • A method for continuously extracting hypoglycemic active ingredients and estrogen active ingredients from Selaginella
  • A method for continuously extracting hypoglycemic active ingredients and estrogen active ingredients from Selaginella

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Embodiment 1

[0026] First, 120g of Selaginella 120g is reflux extracted twice with 1200ml mass concentration of 95% ethanol, each time for 2h, filter and combine the filtrate, concentrate at a temperature of 40°C and a pressure of 0.09MPa, and dry to obtain 5.76g of Selaginella ethanol extract, That is, the blood sugar-lowering active ingredient of Selaginella chinensis of the present invention; then add 1200ml of water to decoct the dregs after ethanol extraction twice, each time for 2 hours, filter and combine the filtrates, concentrate at a temperature of 40°C and a pressure of 0.09MPa, and dry to obtain Selaginella Put 9.39g of water extract of medicinal dregs; put 9.39g of water extract of Selaginella medicinal dregs into a water bath at 80°C to dissolve completely, then add 95% ethanol to make the concentration of ethanol 30%, stir, and stand at 4°C for 12h Overnight, centrifuge at 6000r / min for 10min to obtain the first supernatant; add 95% ethanol to the first supernatant to make th...

Embodiment 2

[0028] First, 240g of Selaginella 240g is extracted twice with 2400ml mass concentration of 95% ethanol under reflux, each time for 2h, filter and combine the filtrate, concentrate at a temperature of 45°C and a pressure of 0.095MPa, and dry to obtain 11.56g of Selaginella ethanol extract, That is, the blood sugar-lowering active ingredient of Selaginella chinensis of the present invention; then add 2400 ml of water to decoct the dregs after ethanol extraction for 2 times, each time for 2 hours, filter and combine the filtrates, concentrate at a temperature of 45°C and a pressure of 0.095 MPa, and dry to obtain Selaginella herb 19.44g of water extract of dregs; 19.44g of water extract of Selaginella medicinal dregs was placed in a water bath at 80°C to dissolve completely, then 95% ethanol was added to make the ethanol concentration 30%, stirred, and stood at 4°C for 20h overnight , centrifuged at 6000r / min for 10min to obtain the supernatant for the first time; added 95% ethan...

Embodiment 3

[0030] First, 360g of Selaginella 360g was reflux extracted twice with 3600ml mass concentration of 95% ethanol, each time for 2h, filtered and combined filtrates were concentrated at a temperature of 50°C and a pressure of 0.1MPa, and dried to obtain 17.21g of Selaginella ethanol extract, That is, the blood sugar-lowering active ingredient of Selaginella chinensis of the present invention; then add 3600ml of water to decoct the dregs after ethanol extraction for 2 times, each time for 2 hours, filter and combine the filtrate, concentrate at a temperature of 50°C and a pressure of 0.1MPa, and dry to obtain a water extract 27.15g; put 27.15g of the water extract of medicinal dregs into a water bath at 80°C to dissolve completely, then add 95% ethanol to make the ethanol concentration 30%, stir, stand at 4°C for 24h overnight, and centrifuge at 6000r / min for 10min , to obtain the supernatant for the first time; add 95% ethanol to the supernatant for the first time to make the eth...

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Abstract

The invention relates to an extraction separation method of female hormone active components from tamariskoid spikemoss herb, which can effectively solve the problem of extraction separation of the female hormone active components from the tamariskoid spikemoss herb for preparing medicines for treating female hormone deficiency. The method comprises the following steps: firstly, refluxing and extracting the tamariskoid spikemoss herb with ethanol twice, and carrying out reduced pressure concentration and drying on the extract to obtain the alcohol extract for later use; then adding water of which the weight is 10 times of that of the original medicinal herb each time to dregs after ethanol extraction, decocting twice for 2 hours each time, merging the water decoction, and carrying out reduced pressure concentration and drying to obtain an aqueous extract; and putting the aqueous extract of dregs into a 80 DEG C water bath to completely dissolve the aqueous extract, then adding ethanol, stirring, standing over night at 4 DEG C, centrifuging, precipitating to obtain supernatant, and carrying out reduced pressure concentration and drying on the supernatant. The method of the invention is simple, scientific, stable and reliable, has simple process, convenient operation, strong process safety, low production cost and few cross components, and is suitable for industrial production in a large scale.

Description

technical field [0001] The invention relates to a method for extracting active ingredients from natural medicines, in particular to a method for continuously extracting hypoglycemic active ingredients and estrogen active ingredients from Selaginella chinensis. Background technique [0002] Selaginella tamariscina (Beauv.) Spring is a perennial herb, which is the whole herb of the Selaginella genus Selaginella in the family Selaginellaceae. "Shen Nong's Materia Medica" records that Selaginella "maintains the evil spirits of the five internal organs, cold and heat pain in women's yin, lumps, blood closure, and lost children", and it is listed as top grade. Physicians of the past dynasties believe that its nature is pungent, and it has the effects of dispelling blood stasis, promoting blood circulation and stopping bleeding. It can be used raw to treat amenorrhea, lumps, bruises, abdominal pain, asthma, and fried charcoal to treat hematemesis, blood in the stool, hematuria, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/11A61P3/10A61P5/30
Inventor 郑晓珂冯卫生李冬梅苏成福王小兰张莉王玮玮
Owner HENAN UNIV OF CHINESE MEDICINE
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