Tissue culturing method for sugarcane seeds
A technology of sugarcane and tissue culture, applied in horticultural methods, botany equipment and methods, gardening, etc., can solve the problems of small growth, degraded varieties, and poor quality of sugarcane, and achieve vigorous growth, strong immunity, and resistance to hydrochloric acid powerful effect
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Embodiment 1
[0015] (1) Under aseptic conditions, the leaf sheaths of Taiwan Sugar No. 27 fine variety sugarcane, the stem tips of sweet bamboo shoots and the leaf bud tips of Stevia rebaudiana were inoculated in conventional MS medium respectively, and detoxified after conventional constant temperature culture for 30 days, Obtain the original seed of the first-generation purified test-tube plantlet respectively;
[0016] (2) Transfer the original seed tissue of purified test-tube plantlets after detoxification to conventional callus-induced MS medium and culture them for 25 days to induce callus. High-voltage electricity, through the electric contact rod, stimulates the culture medium at a speed of 0.1 seconds each time, stimulates once every 180 minutes, stimulates 160 times during the culture period, adjusts the pH of the culture medium to PH 6.0, and makes the purified test tube seedlings original The distant degeneration of this kind of tissue is degenerated callus;
[0017] (3) The ...
Embodiment 2
[0022] (1) Under sterile conditions, use the tender leaves of Taiwan Sugar No. 27 fine variety sugarcane, the stem tips of sweet bamboo shoots and the leaf bud tips of Stevia rebaudiana, respectively, inoculate in conventional MS medium, and detoxify after 35 days of conventional constant temperature culture , to obtain the first-generation purified test-tube plantlet stock;
[0023] (2) Transfer the original seed tissue of purified test-tube plantlets after detoxification to conventional induced callus MS medium and cultivate them for 30 days to induce callus, during which the voltage obtained by the transformer device reaches 12000 volts High-voltage electricity, through the electric contact rod, stimulates the culture medium at a speed of 0.3 seconds each time, stimulates once every 240 minutes, stimulates 150 times during the culture period, adjusts the pH of the culture medium to PH 6.5, and makes the purified test tube seedlings original The distant degeneration of this ...
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