Tissue culturing method for sugarcane seeds

A technology of sugarcane and tissue culture, applied in horticultural methods, botany equipment and methods, gardening, etc., can solve the problems of small growth, degraded varieties, and poor quality of sugarcane, and achieve vigorous growth, strong immunity, and resistance to hydrochloric acid powerful effect

Inactive Publication Date: 2011-12-14
蔡明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for tissue culture of sugarcane species, to solve the various pathogenic bacteria and viruses produced by sugarcane replan

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Under aseptic conditions, the leaf sheaths of Taiwan Sugar No. 27 fine variety sugarcane, the stem tips of sweet bamboo shoots and the leaf bud tips of Stevia rebaudiana were inoculated in conventional MS medium respectively, and detoxified after conventional constant temperature culture for 30 days, Obtain the original seed of the first-generation purified test-tube plantlet respectively;

[0016] (2) Transfer the original seed tissue of purified test-tube plantlets after detoxification to conventional callus-induced MS medium and culture them for 25 days to induce callus. High-voltage electricity, through the electric contact rod, stimulates the culture medium at a speed of 0.1 seconds each time, stimulates once every 180 minutes, stimulates 160 times during the culture period, adjusts the pH of the culture medium to PH 6.0, and makes the purified test tube seedlings original The distant degeneration of this kind of tissue is degenerated callus;

[0017] (3) The ...

Embodiment 2

[0022] (1) Under sterile conditions, use the tender leaves of Taiwan Sugar No. 27 fine variety sugarcane, the stem tips of sweet bamboo shoots and the leaf bud tips of Stevia rebaudiana, respectively, inoculate in conventional MS medium, and detoxify after 35 days of conventional constant temperature culture , to obtain the first-generation purified test-tube plantlet stock;

[0023] (2) Transfer the original seed tissue of purified test-tube plantlets after detoxification to conventional induced callus MS medium and cultivate them for 30 days to induce callus, during which the voltage obtained by the transformer device reaches 12000 volts High-voltage electricity, through the electric contact rod, stimulates the culture medium at a speed of 0.3 seconds each time, stimulates once every 240 minutes, stimulates 150 times during the culture period, adjusts the pH of the culture medium to PH 6.5, and makes the purified test tube seedlings original The distant degeneration of this ...

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PUM

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Abstract

The invention discloses a tissue culturing method for sugarcane seeds. The seed of the general sugarcane is directly the tail in the sugarcane or just the whole sugarcane. If the seeds are kept for a plurality of years, various germs and viruses intrude into the body to cause degeneration of the breed and continuous reduction of the yield. The general method for selecting high-quality seed of the sugarcane is to select variation strains for high-quality seed breeding, so the yield is not increased so greatly. The existing hybrid sugarcane is obtained by hybridizing different well-bred sugarcanes, so the hybrid sugarcane breed is generally hard to embody excellent quality. The tissue culturing method for sugarcane seeds uses cell tissues of bamboo shoots, stevia rebaudiana and sugarcane for cell fusion hybridization, so that the hybrid sugarcane seed prepared by the method disclosed by the invention is characterized by growing strongly, resisting drought, flood, falling, bad environment, diseases and insect pests, and having high immunocompetence. The hybrid sugarcane grows quickly like bamboo shoots and also has the character that the sweet taste of the stevia rebaudianum is better than the sweet taste of the sugarcane, so that the hybrid sugarcane seed prepared by the method disclosed by the invention uniquely has the character of excellent hybrid quality.

Description

technical field [0001] The invention relates to a method for tissue culture of sugarcane species. The high-quality cells of conventional refined sugarcane species are selected for cell fusion with bamboo shoot cells and stevia cells and then cultured to produce a new generation of excellent sugarcane species. Background technique [0002] Conventional sugarcane species are directly reserved for seeding or the whole cane for seeding. If the seeds are reserved for many years, various germs and viruses invade the body and often cause the variety to degenerate, and the yield is getting lower and lower. Conventional sugarcane breeding is to select mutant strains for breeding, and the yield does not increase much. Existing hybrid sugarcanes are hybridized with different fine varieties of sugarcane, and the hybridized sugarcane varieties are often difficult to reflect good quality. [0003] According to the theory of cell theory, it is pointed out that the organs and tissues of hi...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 蔡明
Owner 蔡明
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