A kind of artificial cultivation strain of wild edible fungus and cultivation method thereof
A technology of artificial cultivation of wild edible fungi, applied in the field of artificial cultivation of wild edible fungi, can solve the problems of no artificial cultivation methods, no reports of the discovery of the fungus, etc., to achieve a rich market variety supply, high economic and social benefit effect
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[0023] Side ear of the present invention ( Pleurotus sp. ) P26297 is collected from wild saprophytic fungi in natural forest farms as specimens, and domesticated by tissue separation method.
[0024] During the domestication process, the isolated strains are placed in various environments (different C / N ratios, temperature, pH, light, etc.) for bacterial culture and fruiting, so that the mycelium grows densely, and the mycelia are lined in the later stage. The strain with relatively stable genetic performance was used as the mother species. The specific screening process is as follows:
[0025] The sparse hyphae obtained from tissue separation were cultured for multiple generations at different temperatures, and it was found that at 22°C, the mycelium grew fastest and the mycelium was thick. Through the selection of various carbon sources and nitrogen sources, 1% peptone was added to the basal medium. The tested carbon sources are: maltose, starch, lactose, fructose, sucros...
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