A method for overcoming the vitrification phenomenon of sisal tissue culture seedlings

A technique of tissue culture and vitrification, which is applied to overcome the vitrification phenomenon of sisal tissue culture seedlings. In the field of sisal tissue culture, it can solve the problems of different, not universally applicable, abnormal physiological functions of vitrified seedlings, etc., and achieve loose implementation conditions , The effect of simple implementation steps and easy implementation steps

Inactive Publication Date: 2011-12-21
SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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Problems solved by technology

Usually it is difficult for vitrified plants to return to normal growth during subsequent culture, and vitrified seedlings are still formed in subculture, and the differentiation ability of vitrified seedlings is low, so it is difficult to proliferate, take root and become seedlings
Due to the abnormal physiological function of vitrified seedlings, it is difficult to transplant and survive. Therefore, it is extremely unfavorable to use tissue culture technology to carry out rapid plant propagation in vitro. The problem of vitrification of tissue cultured seedlings has also become an urgent problem in the production of tissue cultured seedlings.
As early as 1981, Debergh et al. conducted a systematic study on the inducing factors of vitrification of tissue culture seedlings. However, because the inducing factors of vitrification of different plants are very different, there are different opinions. There is no universally applicable and effective technical measure to prevent vitrification

Method used

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  • A method for overcoming the vitrification phenomenon of sisal tissue culture seedlings
  • A method for overcoming the vitrification phenomenon of sisal tissue culture seedlings
  • A method for overcoming the vitrification phenomenon of sisal tissue culture seedlings

Examples

Experimental program
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Effect test

Embodiment 1

[0028]Take 15 bulbil seedlings with a height of 8-10cm stored in the sisal germplasm nursery of the South Subtropical Crops Research Institute, remove the roots and old leaves, wash them with running water, treat them with potassium permanganate solution 1000 times for 30 minutes, and then soak them in 75% alcohol for 1 minute , then soak in 0.1% mercuric chloride solution for 15-30 min, and finally rinse with sterile water 3-5 times. After drying, excise part of the explants, inoculate them on the improved SH+6-BA3.0-5.0mg / L medium after longitudinal cutting, and the culture conditions are 12-14 hours of light per day, 10-12 hours of dark culture, light intensity 2000lux, culture temperature is 28±2℃. After 45 days of culture, it was transferred to the bud induction and proliferation medium. The basic medium for bud induction and proliferation was improved SH medium, and the exogenous hormones were 6-BA, NAA and IBA; the concentration of 6-BA was 1.0-5.0 mg / L; the concentrat...

Embodiment 2

[0030] Take 85 clusters (5-8 plants per cluster) of partially or fully vitrified regenerated plants and inoculate them on the modified SH medium, add exogenous hormones as 6-BA, NAA and IBA; the concentration of 6-BA is 1.0-5.0mg / L; the concentration of NAA is 0-1.5mg / L, the concentration of IBA is 0-1.0mg / L, the pH is 5.8-6.0; the light is 12-14h, the dark is 10-12h, the light intensity is 2000Lx, and the temperature is 28±2 ℃. After 1 month of cultivation, 23 clusters had fully recovered to normal growth, 34 clusters had partially recovered, and the recovery rate of vitrified seedlings was 67.06%.

Embodiment 3

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Abstract

The invention relates to a method for overcoming a vitrification phenomenon of sisal hemp tissue culture seedlings. The method comprises the following steps of under an in vitro condition, after utilizing stem tips of sisal hemp to perform surface disinfection for explants, inoculating the explants to an improved SH culture medium which is added with 6-BA to perform primary culture for about 45 days, and then inoculating the aseptic explants to the improved SH culture medium which includes 6-BA to perform induced differentiation and culturing, performing subculture multiplication for the induced cluster bud in each 45-day by utilizing the culture medium with the same induced differentiation, so that a large amount of healthy seedlings can be obtained through rooting culture. The method can be used for controlling the vitrification phenomenon of the tissue culture seedlings by adjusting the effective components of the culture medium, thus, the method is simple, convenient, economic andhighly efficient; producing the tissue culture seedlings by the common sisal hemp using the method can effectively overcome the vitrification phenomenon, therefore, the method has a wide application prospect.

Description

technical field [0001] The invention relates to a method for sisal tissue culture, in particular to a method for overcoming the vitrification phenomenon of sisal tissue cultured seedlings, and belongs to the technical field of tissue cultured seedlings. Background technique [0002] Sisal is an important tropical fiber crop, which is mainly distributed in some tropical and subtropical regions between the Tropic of Cancer and the South. The planting area is very limited and the total amount of resources is scarce; Irreplaceable raw materials such as crane wire rope cores, etc., sisal products have been widely used in metal polishing, special pulp, metal products, transportation, mining, home decoration, carpets, crafts, medicine and automobile industries. [0003] H.11648, the only sisal variety currently planted in my country's sisal production, is an excellent variety introduced from abroad in the 1960s. It has high fiber output and strong cold resistance. It has been widel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 周文钊张燕梅戴梅莲陆军迎李俊峰林映雪
Owner SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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