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Method for producing hydrogen utilizing cellulose

A technology for the production of hydrogen and cellulose, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of high cost, achieve the effects of reducing substrate cost, improving hydrogen production efficiency, and expanding the scope of utilization

Active Publication Date: 2013-08-28
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention solves the problem of high cost of the existing cellulose hydrogen production method, and provides a method for producing hydrogen from cellulose

Method used

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  • Method for producing hydrogen utilizing cellulose

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specific Embodiment approach 1

[0009] Specific embodiment 1: This embodiment is a method for producing hydrogen from cellulose, which is specifically completed according to the following steps:

[0010] 1. Cultivation: Inoculate the spores of Trichoderma viride in a liquid enzyme production medium, and culture it in suspension at 25~35℃ and 120~180r / min aerobic conditions for 3~6 days; 2. Separation: Culture step 1. Centrifuge the liquid enzyme production medium at 3~10℃ for 8~15min at 5000~10000r / min for 3~6 days, and the supernatant I obtained after separation is the crude enzyme solution; 3. Prepare the cellulose solution: crush the cellulose raw material to The particle size is 10~30mm, and it is treated with hot alkali method for two hours, and then added to the citric acid-sodium citrate buffer solution with the substance concentration of 0.05mol / L, and the mass-volume concentration is 20~50g / L cellulose solution; 4. Preparation of cellulose saccharification solution: Add the crude enzyme solution separ...

specific Embodiment approach 2

[0017] Specific embodiment two: this embodiment is different from specific embodiment one in that the Trichoderma viride described in step one is the fungus Trichoderma viride, number: 3.2876, isolation number: Tangyan 37. Others are the same as the first embodiment.

specific Embodiment approach 3

[0018] Specific embodiment three: this embodiment is different from specific embodiment one or two in that: KH in the liquid enzyme production medium described in step one 2 PO 4 The mass-volume concentration is 2.0g / L, (NH 4 ) 2 SO 4 The mass-volume concentration is 1.4g / L, MgSO 4 ·7H 2 The mass-volume concentration of O is 0.3g / L, CaCl 2 The mass-volume concentration of the organic carbon source is 0.3g / L, the mass-volume concentration of the organic carbon source is 30g / L, and the mass-volume concentration of the organic nitrogen source is 5g / L. Others are the same as the first or second embodiment.

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Abstract

The invention provides a method for producing hydrogen utilizing cellulose, relates to a method for producing hydrogen, and is used for solving the problem that the existing hydrogen production methods utilizing cellulose have high cost. The method comprises the following operation steps: 1, culturing; 2, separating; 3, preparing a cellulose solution; 4, preparing a cellulose saccharification liquid; and 5, producing hydrogen. The method provided by the invention has the advantages that: 1, a commercial enzyme is not used, so that the hydrogen production cost is lowered by 60%; 2, the used cellulose generated by Trichoderma viride has a degradation capability to different kinds of celluloses in the natural word, thereby broadening the utilization range of a cellulose raw material; and 3, the yield of hydrogen produced by utilizing the cellulose saccharification liquid is improved by 46 times as compared with the fact that the cellulose solution is directly utilized, thereby improving the hydrogen production efficiency. The method provided by the invention is mainly used for producing hydrogen.

Description

Technical field [0001] The invention relates to a method for producing hydrogen. Background technique [0002] Energy is an important material basis for human survival and sustainable development. The reserves of conventional fossil energy are extremely limited, and the world's proven reserves of oil, natural gas and coal will be depleted in the next 40, 60 and 100 years respectively. At the same time, environmental pollution and climate change caused by the use of fossil energy also pose severe challenges to human survival and development. This makes it very necessary and urgent to develop new, clean and renewable new energy sources. As one of the emerging energy sources, biomass energy is estimated to account for 40% of global energy consumption in the next 40 years. Hydrogen is one of the most easily industrialized varieties of biomass energy. As a new type of clean energy, it has the advantages of a wide range of sources, high combustion value, clean and pollution-free, ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P3/00C12R1/885C12R1/01
Inventor 任南琪赵磊王爱杰曹广丽任宏宇
Owner HARBIN INST OF TECH