Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Breeding Methods for Preventing Gastrodia elata from Degenerating

A technology of gastrodia elata and gastrodin, which is applied in the field of DNA molecular marker selection breeding combined with the content of gastrodin, an effective indicator component of gastrodia, can solve the problems of difficult control of results, limited breeding effect, etc. High yield and high quality results

Inactive Publication Date: 2011-12-21
GUIYANG MEDICAL UNIVERSITY
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the inherent growth characteristics of Gastrodia elata, the breeding time has been as long as more than ten years, and some special traits are still restricted by many conditions. Therefore, the breeding effect of traditional selection of Gastrodia elata is very limited, and the results are difficult to control

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Breeding Methods for Preventing Gastrodia elata from Degenerating
  • Breeding Methods for Preventing Gastrodia elata from Degenerating
  • Breeding Methods for Preventing Gastrodia elata from Degenerating

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Step 1: Rapid analysis of Gastrodia elata germplasm resources

[0077] 1. Extract Gastrodia elata DNA:

[0078] Put the Gastrodia elata tuber or (scape) in a mortar with physiological saline and quartz sand, cool it in a refrigerator at 4°C, quickly grind it into powder, transfer it to a 1.5ml centrifuge tube, add 700μl CTAB lysis buffer preheated at 65°C , mix well, place in a water bath at 65°C for 1-2 hours, shake gently several times during this period, add an equal volume of chloroform / isoamyl alcohol (24:1 V / V), invert several times until no layering occurs, centrifuge at 13000rpm for 15min , transfer the supernatant to another centrifuge tube, add 0.6 times the volume of pre-cooled isopropanol, invert several times until flocculent DNA can be seen, freeze at -20°C for 1 h, centrifuge at 10,000 rpm for 5 min, discard the supernatant, and add 70% ethanol , washed 2 to 3 times, air-dried at room temperature, added 30 to 60 μl TE solution (pH 8.0) until the DNA was ...

Embodiment 2

[0119] Collect different germplasms of Gastrodia elata in Guizhou Province, use AFLP or ISSR, SSR and gastrodin content markers to detect Gastrodia elata at the same time, identify DNA amplification bands with Gel-Pro Analyzer Version software, draw clustering diagrams with NTSYSpc software, and quickly analyze Gastrodia elata germplasm Genetic relationship of resources, draw a map of the relationship between the genetic polymorphism of Gastrodia elata and the content of gastrodin, select the germplasm with high gastrodin content, rich polymorphism, small genetic similarity coefficient, large genetic distance, and long genetic relationship. Identify homozygous and heterozygous, quickly select and determine the parent sowing in the project implementation site: the Gastrodia elata breeding ground of Dafang County, Guizhou Province, Yunlong Gastrodia elata Development Co., Ltd. in Longchangba, Guizhou Province, artificial pollination in the rapid propagation room, and quickly ident...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a breeding method for preventing gastrodia tuber from degradation, which comprises the following steps: detecting gastrodia tuber by simultaneously using AFLP or ISSR, SSR and gastrodine content markers, and drawing a clustering graph and a gastrodia tuber genetic polymorphism / gastrodine content relational graph by using software, thereby quickly analyzing the gastrodia tuber germplasm resource kinship, identifying the gastrodia tuber inbred line and hybrid, quickly selecting and determining parents of the gastrodia tuber, quickly identifying the purity of the gastrodia tuber hybrid, etc., so that the traditional Chinese medicine gastrodia tuber has high quality and is sustainable. The method disclosed by the invention can scientifically, quickly, effectively and progressively solving the problem in the gastrodia tuber seed source, and can select high-quality traditional Chinese medicine gastrodia tuber seeds applicable to local cultivation; under the conditions suitable for intergrowth of germination bacteria and Armillaria mellea as well as scientific management, the yield and quality of the gastrodia tuber seeds are high; and after three generations of reproduction, the content of gastrodine still conforms to the specification in pharmacopoeia.

Description

technical field [0001] The invention belongs to the technical field of planting traditional Chinese medicinal materials, and relates to an artificial breeding method of gastrodia elata, more specifically, to prevent the gastrodia elata from degenerating, a DNA molecular marker selection breeding method combined with the content of gastrodin, an effective index component of gastrodia elata, is adopted. Background technique [0002] Rhizoma Gastrodiae is a precious traditional Chinese medicine, which has been used in my country for more than 2,000 years. Gastrodiae has high medicinal value and economic value. Currently, Tianma Gastrodia elata The artificial cultivation and propagation technology of Bl. is relatively mature. However, in many Gastrodia elata producing areas, after 3 generations of Gastrodia elata propagation, more than 95% of Gastrodia elata grown are elongated or deformed small white and rice hemp, and some rice hemp It is also tangled into agglomerates, with ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 王晓丽郭祥文宋聚先
Owner GUIYANG MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com