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Method for analyzing inhibition of immobilized flow injection enzyme

An immobilized enzyme and flow injection technology, applied in the field of analysis and detection, can solve the problems of unfavorable on-site detection application, time-consuming and cost-consuming, easy introduction of interference, etc., to reduce the source of human interference, save time, and prevent human interference.

Inactive Publication Date: 2012-01-04
QINGDAO UNIV
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Problems solved by technology

Although this analysis method can realize automatic process processing, the detection process includes repeated steps, which require multiple flow path switching, baseline stabilization, and reagent replacement. The process is complex, time-consuming, and easy to introduce interference. The immobilized enzyme that has been artificially passivated and inactivated is placed in the column, which needs to be prepared separately, which consumes extra time and cost, and is inconvenient to carry, which is especially unfavorable for on-site detection applications

Method used

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  • Method for analyzing inhibition of immobilized flow injection enzyme
  • Method for analyzing inhibition of immobilized flow injection enzyme
  • Method for analyzing inhibition of immobilized flow injection enzyme

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Embodiment Construction

[0021] As mentioned above, the present invention provides a flow injection immobilized enzyme inhibition assay method for the analysis of environmental pollutants. The content of the present invention is illustrated by the following example for analyzing the content of dichlorvos pesticide in a sample, but the scope of the present invention is not limited.

[0022] Use a calorimetric biosensor with a loop loop to analyze and determine the concentration of the organophosphorus pesticide dichlorvos in the sample. The calorimetric biosensor system has a twin detection loop and a reference loop. The detection reactor and the reference reactor are Ф7×60mm In a cylindrical reactor, the same batch of immobilized chicken liver lipase was placed in it, the flow rate was 1.5mL / min, the reaction temperature was set at 37°C, pH 6.4, 0.025M citrate buffer was used, and 200mM bottom The enzymatic reaction takes place continuously in the test reactor and the reference reactor.

[0023] Befo...

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Abstract

The invention provides a method for analyzing inhibition of immobilized flow injection enzyme, which comprises the following steps: obtaining the difference between response signals restrained and unrestrained by environmental pollutants and two transition moment parameters in the lowering process of the difference according to an excessive substrate enzyme reaction progress curve by utilizing animmobilized flow injection enzyme analysis system with a circulation loop and obtaining the to-be-detected concentration of the environmental pollutants in light of the analysis to a standard relation curve. The method is simple and quick, has high automation, effectively reduces the artificial interference and can select and correct the immobilized enzyme. The method is applied to the inhibited enzymatic reaction analysis and has a certain advantage in the field monitoring and analysis of pesticide residue, heavy metals and other environmental pollutants.

Description

technical field [0001] The patent of the invention relates to an enzyme inhibition analysis method, especially a flow injection immobilized enzyme inhibition analysis method for analyzing environmental pollutants, which belongs to the field of analysis and detection. Background technique [0002] The impact of environmental pollutants on the environment is becoming more and more serious, especially pesticide residues (organophosphorus and carbamate pesticides, etc.) and heavy metals (mercury, copper, lead, etc.) have become major hidden dangers to human health. Effective detection and monitoring has very important practical significance. [0003] At present, the commonly used detection methods of environmental pollutants include chromatography, enzyme inhibition and so on. In comparison, the enzyme inhibition method is highly accurate, fast and convenient, fast in detection speed, simple in operation, and low in cost. A simple test method for organophosphorus and carbamate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/25
Inventor 郑艺华刘君马永志
Owner QINGDAO UNIV
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