Overhead cultivation method of Dendrobium huoshanense test-tube seedlings
A technology of Dendrobium huoshanense and a cultivation method, applied in the directions of cultivation, soilless cultivation, botanical equipment and methods, etc., can solve the problems of long growth cycle of test-tube seedling production, low survival rate of test-tube seedling transplanting, etc., and achieves simple and easy matrix components. The effect of shortening the production cycle and improving the food safety
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Embodiment 1
[0026] This embodiment takes the non-overhead cultivation method of Dendrobium huoshanense seedlings as a contrast, selects three kinds of cultivation substrates of the inventive method in implementation and carries out the research on the survival rate of Dendrobium huoshanense seedlings after transplanting, and concrete steps are as follows:
[0027] 1. Acquisition of Seedlings
[0028] Select robust flowering plants of Dendrobium Huoshanense, obtain fruit by artificial pollination, and then sow the seeds in the fruit on the seed germination medium for cultivation. After the seeds germinate into protocorms and further differentiate into young seedlings, the seedlings are flowered in test tubes Medium culture, after the cultivated plants bloom, carry out artificial pollination under sterile conditions, cultivate on the artificial medium until the fruit is mature, then carry out aseptic sowing, grow into small plants after the seeds germinate, and finally plant small plants Cu...
Embodiment 2
[0064] 1. Obtaining seedlings
[0065] The method for obtaining seedlings is the same as that in Example 1.
[0066] 2. Facility preparation before seedling transplanting
[0067] According to the method of the present invention, leveling the land, building a shed, placing a three-dimensional frame, erecting a spray equipment and sterilizing the greenhouse 7 days before planting, the specific steps are as follows:
[0068] (1) Leveling the land: remove weeds, level the land, and open drainage ditches around the land;
[0069] (2) Building a shed: build a 5-meter-wide, 2.5-meter-high film greenhouse on the base that can be ventilated and cooled in summer and can be kept warm in winter, and a layer of shading with a shading rate of 60-80% is laid inside and outside the film greenhouse. The net, the inner and outer nets can be opened and closed according to the intensity of the light, so as to adjust the light intensity in the shed, and 40-60 mesh insect nets are set around the...
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