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Rapid propagation method for improving tissue culture seedling quality of Agave sisalana perrine

A technology of tissue culture seedlings and sisal, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem of tropical crop varieties resources of Guangxi Institute of Tropical Crops and Chinese Academy of Tropical Agricultural Sciences for more than 20 years. The Institute and the South Subtropical Crops Research Institute of the Chinese Academy of Tropical Agricultural Sciences have also carried out problems such as limited supply of provenance and long cultivation time, to achieve the effect of solving the degradation of sisal varieties, alleviating the shortage of seedlings, and saving seedling time.

Inactive Publication Date: 2012-01-18
GUANGDONG ZHANJIANG LAND RECLAMATION SCI INST
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AI Technical Summary

Problems solved by technology

The main problems in conventional seedling breeding are: long cultivation time, limited supply of provenance, degeneration of species, mixed good and bad, reduced yield, poor quality, many diseases, and easy to cause adverse factors such as the prevalence of seedling diseases
[0004] As early as the late 1970s, South China Research Institute of Thermal Planting, Fujian Thermal Plantation Institute and Dongfanghong Agricultural Science Institute respectively used sisal leaves, axillary buds and sexual hybrid seeds to induce callus to redifferentiate into complete plants, and obtained a small amount of sisal Tissue culture seedlings, but the survival rate is low in the false planting stage, and the field planting has not been successful
In the following twenty years, the Guangxi Tropical Crops Research Institute, the Institute of Tropical Crops Variety Resources of the Chinese Academy of Tropical Agricultural Sciences, and the South Subtropical Crops Research Institute of the Chinese Academy of Tropical Agricultural Sciences also carried out research on sisal tissue culture technology, and reported the use of sisal. Complete plants were bred from the callus induced by the stem tips and leaves of hemp, but they were not applied in production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] In March 2009, we selected bulbils with a height of 20-30 cm in the shed as explants, cut the head stems into 4 pieces, and rapidly propagated them through tissue culture by directly inducing buds. The medium for inducing adventitious buds is SH + TDZ 1.0mg / L + NAA 0.1mg / L + 5% sucrose + 0.6% agar. After 30 days, the induction rate is 91.1%. 2 pieces, adventitious buds in SH + TDZ0.5mg / L + NAA0.05mg / L + sucrose 5% + agar 0.6% medium, the rate of clustered buds produced after one week was 88.3%. Under the culture conditions of temperature 28±1℃, light 12h / d, light intensity about 2000Lx, and relative humidity about 70%, the vitrification rate is 4.5%. The clustered buds were separated into single buds and inoculated into SH+NAA1.0mg / L+IBA2.0mg / L+activated carbon 0.1%+sucrose 3% medium to induce rooting, and the rooting rate reached 98.3% in about 15 days; After 2-3 months of cultivation, the seedling height is 15-20cm, and then the seedlings are directly planted in bags...

Embodiment 2

[0036]In September 2009, bulbils with a height of 20-30 cm in the shed were selected as explants, and the head and stem were cut into 4 pieces, and the tissue rapid propagation was carried out by directly inducing buds. The medium for inducing adventitious buds is SH+ TDZ 1.0mg / L + NAA 0.1mg / L + sucrose 5% + agar 0.6%. After 30 days, the induction rate is 93.1%, and the adventitious single buds are left with 1cm head stem and cut longitudinally For 2 pieces, adventitious buds in SH + TDZ0.5mg / L + NAA0.05mg / L + sucrose 5% + agar 0.6% medium, after 7 days, the rate of clump buds in adventitious buds was 89.7%. Under the culture conditions of temperature 28±1℃, light 12h / d, light intensity about 2000Lx, and relative humidity lower than 70%, the vitrification rate is 2.5%. The clustered buds were separated into single buds and inoculated into SH+NAA1.0mg / L+IBA2.0mg / L+activated carbon 0.1%+sucrose 3% medium to induce rooting, and the rooting rate reached 99.4% in about 15 days; Af...

Embodiment 3

[0038] In February 2010, bulbils with a height of 20-30 cm were selected as explants, and the head and stem were cut longitudinally into 4 pieces, and rapid tissue propagation was carried out by directly inducing buds. The medium for inducing adventitious buds is SH+ TDZ 1.0mg / L + NAA 0.1mg / L + sucrose 5% + agar 0.6%. After 30 days, the induction rate is 95.5%, and the adventitious single buds are left with 1cm head stem and cut longitudinally For 2 pieces, adventitious buds were placed in SH+TDZ0.5mg / L+NAA0.05mg / L+sucrose 5%+agar 0.6% medium, after 7 days, the rate of clump buds in adventitious buds was 86.2%. Under the culture conditions of temperature 28±1℃, light 12h / d, light intensity about 2000Lx, and relative humidity lower than 70%, the vitrification rate is 3.5%. The clustered buds were separated into single buds and inoculated into SH+NAA1.0mg / L+IBA2.0mg / L+activated carbon 0.1%+sucrose 3% medium to induce rooting, and the rooting rate reached 96.7% in about 15 days; ...

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Abstract

The invention relates to a rapid propagation method for improving tissue culture seedling quality of Agave sisalana perrine. The method comprises the steps of: (1) explant treatment and disinfection; (2) initiation and inoculation; (3) induction of adventitious bud cultivation; (4) induction of clumpy bud cultivation; (5) multiplication of subculture; (6) rooting culture; (7) seedling adaptation;(8) temporary planting and transplanting; (9) bagged dispersal breeding seedling cultivation. The method of the invention adopts SH as a basic medium and more active cytokinin TDZ (thidiazuron), in cooperation with other cultivation conditions. The method provided in the invention also employs a different tissue culture seedling cutting method and changes the original seedling culture method of Agave sisalana perrine, so that the seedling culture time can be saved by 6 months and the problems like variety degeneration and sickness of Agave sisalana perrine can be solved effectively, thus effectively guaranteeing seedling quality and promoting health development of the Agave sisalana perrine industry.

Description

technical field [0001] The invention relates to a method for sisal tissue culture seedlings, in particular to a rapid propagation method for improving the quality of sisal tissue culture seedlings, which belongs to the technical field of tissue culture. Background technique [0002] Sisal ( Agave sisalana perrine ) of the Agaveaceae ( Agavaceae ) Agave ( Agave ), perennial monocotyledonous herbaceous hard fiber crops. Sisal has a growth cycle of more than 12 years, high economic value, and wide comprehensive uses. It is a hard fiber with the largest amount and the widest range in the world today. It has the characteristics of strong tensile force, sea immersion resistance, friction resistance, high elasticity, and not easy to slip, which cannot be replaced by synthetic fibers. It can be made into ropes for fishing, navigation, mining, transportation, etc. It can be woven into sacks, wallpaper cords, carpets, and battings. It can also be mixed with plastics and pressed ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 姜伟揭进刘伟清李愿平李强有
Owner GUANGDONG ZHANJIANG LAND RECLAMATION SCI INST
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