Solid-phase carrier for surface fixation and preparation and application methods for solid-phase carrier
A technology of solid-phase carrier and surface immobilization, applied in solid-phase fermentation bioreactors, methods of supporting/immobilizing microorganisms, specific-purpose bioreactors/fermenters, etc., can solve the problem of harsh detection signal conditions and solid-phase surface immobilization Problems such as too few groups, to achieve the effect of low production cost, lower requirements, and simple operation process
Active Publication Date: 2014-05-07
盛司潼
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Problems solved by technology
[0006] The purpose of the present invention is to provide a solid phase carrier for surface immobilization and its preparation method and application, aiming to solve the problem of too few immobilized groups on the surface of the solid phase and too harsh detection signal conditions, and at the same time simplify operations and reduce production costs. cost
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[0048] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.
[0049] A solid-phase carrier for surface immobilization, the solid-phase carrier includes a glass slide and at least one surface group with an activation function; the surface group is directly connected to the glass slide, including a fixed end and an active end, and its molecular The structural formula is as follows:
[0050]
[0051] Wherein, the fixed end has at least one siloxy group for combining with the glass slide.
[0052] figure 1 The general structure of the surface group in the solid phase carrier used for surface immobilization in the present invention is shown as follows:
[0053]
[0054]Wherein, the surface group includes a fixed end and an active end, and the fixed end has at least one siloxyl group for connecting with the...
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The invention relates to the field of surface chemical modification and genetic engineering and provides a solid-phase carrier for surface fixation and methods for preparing and applying the solid-phase carrier. The solid-phase carrier for surface fixation is connected with at least one surface group with an activation function, wherein the surface group contains a fixation end and an active end; and the molecular structural formula of the surface group is shown in the description: the fixation end is provided with at least one silyloxy group for combining the solid-phase carrier and the active end -R contains an active functional group for combining a surface fixation connection. In the invention, the solid-phase carrier is used for activation, and then resin is used for embedment and the solid-phase carrier is re-activated so as to ensure that the surface group on the surface of the solid-phase carrier is doubled, so that the quantity of the surface fixation connections fixed on the solid-phase carrier is increased.
Description
technical field [0001] The invention relates to the fields of surface chemistry and genetic engineering, more specifically, to a solid phase carrier for surface immobilization, a preparation method of the solid phase carrier, and an application method of the solid phase carrier. Background technique [0002] DNA sequencing technology has been widely used in various fields of biological research, many biological problems can be solved with the help of high-throughput DNA sequencing technology, and massively parallel DNA sequencing platform (massively parallel DNA sequencing platform) has developed into the mainstream sequencing technology . At present, the more advanced sequencers in the world use a new sequencing strategy - cyclic-array sequencing (cyclic-array sequencing), which can also be called "next-generation sequencing technology or second-generation sequencing technology". [0003] Most of the next-generation sequencing methods obtain parallel sequencing templates t...
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IPC IPC(8): C12Q1/68C12M1/00
CPCC12M21/16C12M25/16
Inventor 盛司潼
Owner 盛司潼