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Microbial strain and deodorant for municipal landfill, and preparation and application method thereof

A technology of municipal solid waste and microorganisms, which is applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high production input and product cost, continuous product quality and use effect, and unstable ratio of effective flora, etc. The problem is to reduce the chance of contamination, shorten the fermentation cycle, and achieve the effect of good deodorization.

Active Publication Date: 2013-08-28
HUNAN INST OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some components are complex, the proportion of effective bacteria is not stable, which leads to constant problems in product quality and use effect
Moreover, different fermentation methods are used to produce different strains, resulting in very expensive production inputs and product costs.

Method used

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  • Microbial strain and deodorant for municipal landfill, and preparation and application method thereof
  • Microbial strain and deodorant for municipal landfill, and preparation and application method thereof
  • Microbial strain and deodorant for municipal landfill, and preparation and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Prepare PDA medium and MRS medium, and sterilize at 121°C for 15 minutes. Subsequently, PDA was inoculated with Saccharomyces cerevisiae CGMCC No.5183, and cultured for 20 h at a culture temperature of 28° C. to 30° C. and a rotation speed of 180 rpm to obtain a yeast seed liquid. Lactobacillus casei CGMCC No.5182 was inoculated in MRS medium, and cultured at 35° C. to 37° C. for 30 hours to obtain Lactobacillus seed liquid.

[0034] Preparation of fermentation medium: 10% molasses, 3% bean sprout juice, 1% wheat bran, 0.1% yeast extract, 0.1% peptone, the rest is water, pH 5.0-5.5; sterilize in a fermenter at 121°C for 15 minutes ;

[0035] Insert the Saccharomyces cerevisiae seed liquid accounting for 1% of the medium volume into the sterilized fermentation medium that accounts for 50% to 70% of the volume of the fermentation tank. The culture temperature is 28°C, the tank pressure is 0.01~0.03MPa, and the air is passed first. Stir, the ratio of aeration volume to f...

Embodiment 2

[0040] Prepare PDA medium and MRS medium, and sterilize at 121°C for 15 minutes. Subsequently, Saccharomyces cerevisiae was inoculated into the PDA, and cultured for 20 hours at a culture temperature of 28° C. to 30° C. and a rotation speed of 180 rpm to obtain a yeast seed liquid. Lactobacillus casei was inoculated in MRS medium, and cultured at 35°C to 37°C for 30 hours to obtain Lactobacillus seed liquid.

[0041] Preparation of fermentation medium: 10% molasses, 3% bean sprout juice, 1% wheat bran, 0.1% yeast extract, 0.1% peptone, the rest is water, pH 5.0-5.5; sterilize in a fermenter at 121°C for 15 minutes .

[0042] Insert the Saccharomyces cerevisiae seed liquid accounting for 2% of the medium volume into the sterilized fermentation medium that accounts for 50% to 70% of the volume of the fermentation tank. The culture temperature is 30°C, the tank pressure is 0.01~0.03MPa, and the air is passed first. Stir, the ratio of aeration volume to fermentation broth volume...

Embodiment 3

[0047] Prepare PDA medium and MRS medium, and sterilize at 121°C for 15 minutes. Subsequently, Saccharomyces cerevisiae was inoculated into the PDA, and cultured for 20 hours at a culture temperature of 28° C. to 30° C. and a rotation speed of 180 rpm to obtain a yeast seed liquid. Lactobacillus casei was inoculated in MRS medium, and cultured at 35°C to 37°C for 30 hours to obtain Lactobacillus seed liquid.

[0048] Preparation of fermentation medium: 10% molasses, 3% bean sprout juice, 1% wheat bran, 0.1% yeast extract, 0.1% peptone, the rest is water, pH 5.0-5.5; sterilize in a fermenter at 121°C for 15 minutes .

[0049] Insert Saccharomyces cerevisiae seed liquid accounting for 3% of the volume of the medium into the sterilized fermentation medium that accounts for 50% to 70% of the volume of the fermentation tank. The culture temperature is 32°C, the tank pressure is 0.01 to 0.03MPa, and the air is passed first. Stir, the ratio of aeration volume to fermentation broth ...

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Abstract

The invention provides a microbial deodorant and strain for eliminating waste odor of municipal landfill and waste transfer station, and the preparation and application method thereof. The preparation method comprises the following steps: culturing Saccharomyces cerevisiae with the conservation number of CGMCC No.5183 in a PDA culture medium, and culturing Lactobacillus casei (CGMCC No.5182) in an MRS culture medium to obtain the seed liquid; and fermenting in a fermentation tank to obtain the microbial deodorant. The microbial deodorant is prepared by a mixed culture manner so as to lower the fermentation cost, reduce the generation of mixed bacteria and stabilize the product quality. The microbial deodorant prepared by the method can effectively eliminate the waste odor of the municipallandfill so as to achieve good deodorant effect.

Description

technical field [0001] The invention relates to a microbial deodorant, a bacterial strain and a preparation method thereof, in particular to a microbial deodorant, a bacterial strain and a preparation method thereof for eliminating peculiar smells in municipal solid waste landfills and garbage transfer stations. Background technique [0002] With the acceleration of my country's urbanization process, the urban population is increasing day by day, followed by the rapid increase of urban domestic waste. According to incomplete statistics, in recent years, the annual growth rate of my country's garbage has reached more than 10%, and nearly 150 million tons of garbage are generated every year. At present, domestic municipal solid waste has accumulated as much as 7 billion tons, of which 80% to 90% come from large and medium-sized cities, but the treatment rate is only about 50%. Among the more than 600 cities in the country, two-thirds of the large and medium-sized cities have ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L9/01C12N1/20A61L101/52C12R1/865C12R1/245
Inventor 许丽娟贺月林张德元尹红梅王震陈薇吴迎奔丁祥力
Owner HUNAN INST OF MICROBIOLOGY
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