Biological prevention bacterial strain BS1 (bacillus cereus) for preventing and controlling greenhouse cucumber downy mildew and application thereof

A cucumber downy mildew, greenhouse technology, applied in the direction of application, bacteria, biocide, etc., to achieve the effect of saving expenses and increasing income

Inactive Publication Date: 2012-01-25
HUAIYIN TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the first report of cucumber downy mildew resistance to metalaxyl in 1979, subsequent reports on its resistance to fofosalum appeared, and cross-resistance to metalaxyl-resistant downy mildew strains was also found

Method used

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  • Biological prevention bacterial strain BS1 (bacillus cereus) for preventing and controlling greenhouse cucumber downy mildew and application thereof
  • Biological prevention bacterial strain BS1 (bacillus cereus) for preventing and controlling greenhouse cucumber downy mildew and application thereof
  • Biological prevention bacterial strain BS1 (bacillus cereus) for preventing and controlling greenhouse cucumber downy mildew and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Strain source: BS1 was isolated from banana rhizosphere soil in Mayong, Dongguan City, Guangdong Province.

[0025] Strain Isolation Method: Plate Dilution Method (Microbiological Laboratory of Nanjing Soil Institute, Chinese Academy of Sciences. Soil Microbial Research Method [M]. Beijing: Science Press Du, 1985.)

[0026] Screening basis for strains: detection of the activity of five enzymes in the strain hydrolysis (protease, chitinase, cellulase, siderophilic, IAA determination of Yang J.-H., Liu H.~X., Zhu G-M., Xu L. .-P., Pan Y.-L., and Guo J.-H.*.2008.Diversity analysis of antagonists from rice associated bacteria and their application in biocontrol of rice diseases.Journal of Applied Microbiology.104(1): 91~104.) and antagonism against Phytophthora (confrontation culture method, Lin Fucheng, Li Debao. Lysis of Bacillus subtilis to plant pathogenic fungi [J]. Phytopathology Acta, 2003, 33( 2): 174-177.), through the evaluation of bacteria (Faltin et al., 2004),...

Embodiment 2

[0031] Embodiment 2 bacterial agent preparation

[0032] (1) Cultivation of seed bacteria liquid: Inoculate BS1 (CGMCC NO.4736) into LB culture liquid, culture at 32°C and 200 rpm for 18-24 hours, and after 16 hours, take samples at 600nm every 3 hours in the ultra-clean workbench. OD value, during the measurement process, use the uninoculated culture solution to adjust to zero, until the OD value of the seed bacteria solution is between 0.5 and 0.8, the culture is ended, and the seed bacteria solution is obtained;

[0033] (2) Preparation of wet bacteria: Add the seed bacteria solution into a fermenter equipped with 200L fermentation broth at a volume ratio of 1:100, shake and cultivate at 250 rpm at 30°C for 20 to 24 hours, maintain the pH value of the fermentation broth at 7.0, and the dissolved oxygen 20%, ventilation volume 6m 3 / hour, tank pressure 0.05 ~ 0.1KPa. Then 6000rpm centrifugal 10min, every 1000ml culture solution obtains 12~15g wet bacteria, gets wet bacter...

Embodiment 3

[0035] Example 3 Indoor sporangia germination test

[0036]Method: slide culture method (references: NY / T 1156.1-2006 Pesticide Indoor Bioassay Test Guidelines Fungicide Part 1: Inhibition of pathogenic fungus spore germination test concave slide method): the bacterial strain BS1 ( CGMCC NO.4736) is that the biocontrol bacterium agent of effective bacterium is formulated into corresponding concentration, is dripped respectively on the sterilized glass slide, then drops 10 μ l downy mildew sporangia suspension prepared in the bacterial liquid, concentration is 10 5 sporangia / ml, 4 repetitions for each treatment, with sterilized water as a control, placed in a light incubator at 25°C for 48 hours to observe the germination rate of sporangia, microscopic examination was based on 30 sporangia in the field of vision, and observed The standard is that the germ tube length is greater than 1 / 2 of the sporangia width of downy mildew bacteria, and the calculation formula is inhibition ...

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Abstract

The invention belongs to the technical field of plant protection, and relates to a biological prevention bacterial strain BS1 (bacillus cereus) for preventing and controlling greenhouse cucumber downy mildew and an application thereof. The bacterial strain BS1 is bacillus cereus and is collected in the China General Microbiological Culture Collection Center with the collection number of CGMCC NO.4736. The biological prevention bacterial strain BS1 for preventing and controlling greenhouse cucumber downy mildew takes the bacillus cereus of which the collection number is CGMCC NO.4736 as an effective ingredient, wherein the viable bacteria concentration of the BS1 is 1*10<9>-10<10>CFU (colony-forming unit) / ml. After being diluted by 100 times, the biological prevention bacterial strain is sprayed after the cucumber is transplanted for 20d, and the investigation shows that the biological prevention effect on the cucumber downy mildew is 60.64% after 8d; and the biological prevention effect is 65.43% when spraying and root irrigation are simultaneously carried out. The bacillus cereus BS1 can be used for preparing the pesticide capable of preventing and controlling the greenhouse cucumber downy mildew.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and relates to a biocontrol bacterial strain BS1 for preventing and treating cucumber downy mildew in greenhouses and an application thereof. Background technique [0002] Cucumber is one of the top ten vegetable cultivated crops in the world, and occupies an important position in my country's "vegetable basket" project. There are many diseases of its leaves, among which downy mildew is the most harmful. Cucumber downy mildew, commonly known as "Happy Horse Dry", is a fungal disease caused by the flagellate fungus Psedoperonspora cubensis (Berkis Curt) Rostov, which occurs widely in various places. The sporangia of the fungus can be transmitted by wind and rain, cucumber beetles, agricultural implements, etc., and it is a major airborne disease; and it is an obligate parasite with strong specialization selectivity for host plants. [0003] With the sharp increase in the area of ​​green...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/085
Inventor 罗玉明郑丽李师默杨威
Owner HUAIYIN TEACHERS COLLEGE
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