Angiotensin converting enzyme (ACE) inhibitor

A mixture, protease hydrolysis technology, applied in the fields of cardiovascular system diseases, organic chemistry, drug combination, etc., can solve the problem of no ACE inhibitor reports etc.

Inactive Publication Date: 2012-02-01
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] So far, there is no report on the p...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 1. Preparation of Starworm Polypeptide

[0030] 1.1. Extraction of starworm protein

[0031] According to the conventional protein extraction method, use low concentration NaCl solution to extract the star worm protein, as follows:

[0032] Clean and dissect freshly collected star worms, remove viscera and a small amount of visible fat (if not used immediately, it can be stored at -20°C), then soak in 95% ethanol for 3 hours while stirring continuously to fully degrease; then Rinse the defatted starworms with deionized water to wash away the ethanol. Then the sample was mixed with 1% NaCl solution in a ratio of 1:20 to make a pulp, and the tissue was broken, and then the cells were further broken by ultrasonication for 25 minutes, so that the protein in the star worm was fully dissolved in the low-salt solution, and the extraction temperature was controlled at 50°C, extracting for 4h under constant stirring. The protein content in the supernatant was measu...

Embodiment 2

[0042] Embodiment 2, preparation of starworm polypeptide by direct hydrolysis of starworm body

[0043] Wash the freshly collected star worms, beat the tissue directly, and break the cells by ultrasonic, adjust the pH of the slurry to 2.0, the temperature is 37°C, then add 1% pepsin, and hydrolyze it under constant stirring, and add 1M NaOH to adjust the pH during the process , keep the pH constant, finish the reaction after 12 hours of hydrolysis, boil for 10 minutes to inactivate the enzyme, centrifuge, take the supernatant, adjust the pH to 7, concentrate the protein hydrolyzate, and dry to obtain the starworm hydrolyzate.

Embodiment 3

[0044] Example 3, preparation of ACE inhibitors by hydrolysis of starworm dry powder

[0045] In view of the star worm harvest season, there will be a large number of star worms that will not be processed in time. Therefore, the star worms can be dried and made into dried star worms for storage. Grind the preserved star worms and pass through a 100-mesh sieve to obtain star worm dry powder; weigh 300 mg of the dry powder, add 200 mL of deionized water, and mix; then adjust the temperature and pH to 37 ° C and pH 2, respectively, and add pepsin 0.2% (w / v) Carry out the enzymatic hydrolysis reaction, stirring continuously during the process, and adding 1M NaOH to adjust the pH, keep the pH constant, finish the reaction after 24 hours of reaction, boil the reaction solution in a boiling water bath for 10 minutes to kill the enzyme, adjust the pH to neutral, and then cool , centrifuge, and filter to remove denatured precipitates. The protease hydrolyzate is subjected to ultrafil...

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Abstract

The invention discloses polypeptide of Phascolosoma esculenta which is a polypeptide segment prepared through hydrolysis of extracted protein of the Phascolosoma esculenta. The protein of the Phascolosoma esculenta comprises water-soluble protein, waterinsoluble protein and a mixture thereof. The hydrolysis method comprises protease hydrolysis, acid hydrolysis and alkali hydrolysis. The polypeptide of the Phascolosoma esculenta can be used for inhibiting angiotensin converting enzyme (ACE), so the polypeptide can be used for preparing antihypertensive medicines, and a new way for effectively utilizing the Phascolosoma esculenta is opened up.

Description

technical field [0001] The invention belongs to the technical field of food and medicine, in particular, it relates to an inhibitor of angiotensin-converting enzyme (ACE) from acanthus. Background technique [0002] Phascolosoma esculenta, Sipunculus nudus, commonly known as sea maggots, sandworms, soil bamboo shoots, etc., is an indigenous species in my country, distributed in the coastal areas south of the Yangtze River Estuary, inhabiting intertidal zones and mudflats in high tide areas. Benthic cave life. It not only has crisp and tender meat and delicious taste, but also has high medicinal and therapeutic value, and can regulate various functions of the body. Chinese Pharmacopoeia records its edible and medicinal value. It is cool in nature and cold in taste. It returns to the lungs and kidney meridian. It has the effect of nourishing yin and reducing fire. Substitutes are used to treat night sweats due to yin deficiency, hot flashes due to bone steaming, tuberculosis ...

Claims

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Application Information

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IPC IPC(8): C07K2/00C07K1/12C12P21/06A61K38/01A61P9/12
Inventor 谢静莉方明杜磊吴燕玲魏东芝
Owner EAST CHINA UNIV OF SCI & TECH
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