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Process for making improved chromatography media and method of use

A chromatographic method and an adsorbent technology, applied in the field of preparation of the medium, can solve the problems of not providing ligands and the like

Inactive Publication Date: 2012-02-08
ROHM & HAAS CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method allows ligands to be coated on the surface of particles, including beads, but does not provide ligands that protrude into the pores to provide high capacity for biomolecules

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 - Preparation of polyligands

[0041] Weigh 0.111 g of 2-aminoethanethiol hydrochloride (AET·HCl) and 0.186 g of 2,2'-azobis(2-methylpropionamidine) dihydrochloride (ABMPA·2HCl) in a separate weighing dish , added in a 250-ml round-bottomed flask, rinsed with 38 grams of water. 30 grams of (3-acrylamidopropyl)trimethylammonium chloride (AAPTMAC) and 32 grams of water were directly weighed into the flask. Put the thermometer and water condenser (with N 2 Bubbler) is connected and installed with the flask. The components were added to the flask, and the reaction mixture was stirred at 78°C for 5 hours.

Embodiment 2

[0042] Example 2 - Formation of Absorbent Material (Reaction of Polyligand with Polymeric Carrier)

[0043] 90 grams of cooled polymer ligand solution from Example 1 and 10.6 grams of polyGMA-GlyDMA wet cake were added to the round bottom flask. in N 2 The slurry solution was stirred with an overhead stirrer (200 rpm) at 65°C for 16 hours under atmosphere. The resulting cooled suspension was filtered, washed with water (4X200 mL). Anion exchange resins are stored as a slurry in aqueous solution. The anion exchange resin has a bovine serum albumin (BSA) capacity of 155 mg / ml.

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PUM

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Abstract

The present invention is directed to the process for making a macroporous polymeric media with covalently bonded quaternary ammonium polymer chains. The media can be applied for chromatographic purification, resulting in porous substrates having improved protein binding capacity and resin selectivity. The invention also relates to methods relating to making and using the media.

Description

technical field [0001] The present invention relates to a process for preparing porous polymeric media having covalently bonded polymer chains. The medium can be used for chromatographic purification to obtain a porous substrate with improved protein binding capacity, kinetic properties and selectivity, and the invention also relates to methods for preparing and using the medium. Background technique [0002] Therapeutic proteins derived from living organisms are of increasing importance in modern health care. These proteins offer many advantages over conventional drugs, including improved specificity and efficacy for the target disease. The mammalian immune system uses many proteins to control and eliminate disease threats. Advances in genetic and protein engineering have enabled the development of many "designed" or recombinant protein therapeutics. These therapies may be based on individual proteins, chemically modified proteins, protein fragments or protein combinatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/285B01J20/30B01D15/08
CPCB01J39/26B01J41/20B01J20/3278B01J20/26B01J20/286B01D15/36B01J20/285B01J20/30C07K1/18
Inventor M·J·迪兹J·J·迈克纳
Owner ROHM & HAAS CO