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Novel application of Spondin-2 (SPON2)

A technology for spinal protein and prostate cancer, applied in the direction of material inspection products, measuring devices, instruments, etc., can solve problems such as difficulty in distinguishing benign lesions from malignant lesions of the prostate, unsatisfactory results, and elevated serum PSA

Inactive Publication Date: 2014-03-26
INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, it has been found that the specificity of PSA detection for prostate cancer is poor, and the serum PSA of patients with benign prostatic hyperplasia and prostatitis will also increase
In addition, the serum PSA level between 4 and 10ng / ml belongs to the gray area, and it is difficult to distinguish benign and malignant lesions of the prostate
There are still some markers, or the clinical application is relatively limited, or the effect is not very satisfactory

Method used

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  • Novel application of Spondin-2 (SPON2)
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  • Novel application of Spondin-2 (SPON2)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Embodiment 1, the kit for diagnosing prostate cancer

[0096] In the present embodiment, the content value of SPON2 or PSA in the detection sample all uses M (Q R ) represents, M represents the median of SPON2 content in the sample; Q R Indicates the interquartile range of the SPON2 content in the sample, that is, Q R = the absolute value of the difference between the upper and lower quartile values.

[0097] 1. Kit composition

[0098] 1. Coating antibody: polyclonal antibody against Spinin 2 from sheep (SPON2 sheep polyclonal antibody).

[0099] 2. Binding antibody: mouse-derived monoclonal antibody against Spinin 2 (SPON2 mouse monoclonal antibody).

[0100] 3. Standard product: spinal protein 2. The blocking solution and the spinal protein 2 were composed of the following solutions with different concentrations of the spinal protein 2: 100, 50, 25, 12.5, 6.3, 3.1 and 1.6 ng / ml.

[0101] 4. Coating buffer: composed of sodium carbonate, sodium bicarbonate and wa...

Embodiment 2

[0161] Embodiment 2, the expression of SPON2 in cells

[0162] The expression of SPON2 in various prostate cancer cell lines was detected by semi-quantitative RT-PCR. The expression of SPON2 in various prostate cancer cell lines was detected by Western Blot of extracellular protein.

[0163]BPH is a model of benign prostatic hyperplasia. LNCaP and C4-2 cells are internationally recognized cell models for prostate cancer research. PC3 is a cell model of prostate cancer bone metastasis, androgen receptor negative; C4-2B is a cell line of bone metastasis evolved from LNCaP, which also has androgen-independent properties and is AR-positive; DU145 is a prostate cancer cell line A cell line with brain metastases, androgen receptor negative.

[0164] The result is as Figure 4 and Figure 5 shown. The results showed that SPON2 was specific to prostate cancer cell lines and was only expressed in AR-positive prostate cancer cell lines.

Embodiment 3

[0165] Embodiment 3, immunohistochemical method diagnosis prostate cancer

[0166] 1. The immunohistochemical staining analysis (tissue microarray) method of SPON2 in prostate tissue is as follows:

[0167] 1. Baked slices, then dewaxed to water: xylene I 20min, xylene II 20min, 100% ethanol 5min, 100% ethanol 5min, 95% ethanol 5min, 90% ethanol 5min, 85% ethanol 5min, 75% ethanol 5min, Deionized water 5min;

[0168] 2. Antigen retrieval with citrate restoration solution;

[0169] Citrate Repair Solution Storage Solution:

[0170] A.0.1M citric acid solution: weigh 21.01g citric acid (C 6 h 8 o 7 ·H 2 O) Dissolve in 1000ml distilled water.

[0171] B.0.1M sodium citrate solution: weigh 29.41g sodium citrate (C 6 h 5 Na 3 o 7 2H 2 O) Dissolve in 1000ml distilled water.

[0172] Citrate repair solution working solution: Take 9ml of solution A and 41ml of solution B and add it to 450ml of distilled water, the pH value of the solution should be 6.0).

[0173] 3. Afte...

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Abstract

The invention discloses novel application of Spondin-2 (SPON2). The novel application is application of an antibody for the SPON2 in preparation of a kit for aided diagnosis of prostatic cancer. An experiment shows that: an enzyme coupled immune kit for detecting the SPON2 or an immunohistochemistry kit for detecting the SPON2 are used for diagnosing the prostatic cancer, so a diagnosis result is more precise and accurate; and the novel application is superior to the conventional prostatic specific antigen (PSA) diagnosis method.

Description

technical field [0001] The present invention relates to a new application of SPON2. Background technique [0002] Prostate cancer is one of the most common malignant tumors among men in North American countries. In 2008, the newly diagnosed patients with prostate cancer in the United States accounted for 25% of the newly diagnosed patients with malignant tumors in that year. In my country, with the gradual aging of the population structure, the incidence of prostate cancer is also increasing year by year. [0003] Tumor serum markers are the hotspots of current research. An ideal tumor serum marker should have the following characteristics: it can distinguish between high-risk and healthy groups, it can be used for tumor diagnosis, the method is simple and low-cost, it can be used for clinical stage evaluation of tumors, and it can be used for evaluation of tumor treatment effects. It can be used to predict tumor metastasis and recurrence. [0004] PSA is currently the mo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N33/577
Inventor 周建光钱晓龙
Owner INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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