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Plant estrogenic effect of acanthopanax biochemical traditional Chinese medicine compound extract and its application

A technology of extracts and traditional Chinese medicines, applied in the field of preparation of pharmaceutical preparations and health food for the treatment of perimenopausal syndrome, can solve the problem of no phytoestrogen-like effect, different preparation processes, no treatment of women's perimenopausal syndrome, etc. question

Active Publication Date: 2012-03-07
DUODUO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Wujia biochemical medicine mentioned in CN 1415363A is similar to the prescription of the present invention, but the preparation process is different, and there is no phytoestrogen-like effect in its mentioned functions and indications, and it does not treat women's perimenopausal syndrome. The role of disease

Method used

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  • Plant estrogenic effect of acanthopanax biochemical traditional Chinese medicine compound extract and its application
  • Plant estrogenic effect of acanthopanax biochemical traditional Chinese medicine compound extract and its application
  • Plant estrogenic effect of acanthopanax biochemical traditional Chinese medicine compound extract and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] TQW's estrogen-like effect in vivo experimental research adopts three classic animal models recognized internationally for the study of estrogen effects: immature mice, ovariectomized mice, and aging rat models respectively simulate the three physiological stages of low estrogen in women's life.

[0051] 1. Effect of TQW on target organs of immature mice

[0052] The immature mice are just weaned mice at 21 days after birth. At this time, the mice have not yet reached sexual maturity and body maturity. Drugs with estrogen-like effects can promote the development and maturity of their reproductive system.

[0053] 1 Materials and methods

[0054] 1.1 Materials

[0055] KM mice, ♀, 21 days old, were purchased from the GLP Center of Heilongjiang University of Traditional Chinese Medicine.

[0056] TQW suspension: suspend TQW in distilled water, and the ig dose is 15 mg / kg.

[0057] Nilestriol suspension (Nilestrdiol): nilestriol (purchased from Hualian Pharmaceutical Fa...

Embodiment 2

[0163] MCF-7 cells are ER-positive human breast cancer cell lines, which can be specifically regulated by estrogen or estrogen-like active substances to proliferate. The MCF-7 cell estrogen-like proliferation assay is very sensitive, simple and easy to perform, and is widely used in the rapid screening and evaluation of phytoestrogens.

[0164] 1 method

[0165] 1.1 The effect of TQW on the proliferation of MCF-7

[0166] 1.1.1 Cell culture

[0167] MCF-7 cells were cultured in DMEM high-glucose solution containing 10% fetal bovine serum at 37°C, 5% CO 2 , the relative saturation humidity.

[0168] 1.1.2 MTT cell proliferation assay

[0169] Two days before the start of the experiment, the cells were washed twice with PBS, and then cultured in phenol red-free DMEM (containing 5% CD-FBS) to deplete the estrogen stored in the cells. Select the cells in the logarithmic growth phase, digest the cells with 0.25% trypsin, and divide 2×10 3 cells / well were inoculated on a 96-we...

Embodiment 3

[0179] To observe the effect of TQW on the related symptoms of perimenopausal syndrome such as hot flashes, insomnia and hyperlipidemia.

[0180] 1. Effect of TQW on adrenal weight and rectal temperature of ovariectomized mice

[0181] Using ovariectomized mouse experiments, ovariectomy can rapidly reduce the level of estrogen in the body, which can simulate the physiological and pathological characteristics of postmenopausal women.

[0182] 1 Materials and methods

[0183] 1.1 Materials

[0184] KM mice, ♀, 12 weeks old, non-pregnant, were purchased from the GLP Center of Heilongjiang University of Traditional Chinese Medicine.

[0185] TQW suspension: suspend TQW in distilled water, and the ig dose is 15 mg / kg.

[0186] Nilestriol suspension (Nilestrdiol): nilestriol (purchased from Hualian Pharmaceutical Factory of Shanghai Pharmaceutical Group, National Drug Approval: H3102164), suspended in distilled water, ig 0.5 mg / kg.

[0187] 1.2 Method

[0188] 1.2.1 Preparation...

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Abstract

The invention relates to a traditional Chinese medicine compound extract with plant estrogenic effect. The traditional Chinese medicine compound extract takes six traditional Chinese medicines of acanthopanax, Chinese angelica, hemlock parsley, peach seed, dry ginger and licorice as raw materials, a carbon dioxide supercritical extraction technology and a macroporous adsorption resin enrichment technology are employed, the extract is prepared under certain technological parameter. The extract has obvious plant estrogenic effect, enables bidirectional adjustment of organism estrogenic level, prolongs the arrival of climacterium, and is suitable for improving the perimenopausal syndrome of female, such as menstrual disorder, vasomotorial symptom (flush, hot flash), mental and nervous disorder (depression, anxiety and easy excitement and insomnia), bone and joint symptom (osteoporosis, arthralgia and the like), urogenital tract symptom and the like. The extract can be directly and safelyused for preparing the medicinal preparation and health food for treating perimenopausal syndrome.

Description

technical field [0001] The present invention relates to a compound extract of traditional Chinese medicine, in particular to a carbon dioxide supercritical extraction technology and macroporous adsorption resin enrichment technology, using angelica, chuanxiong, peach kernel, dried ginger (cannon), licorice and eleuthero as raw materials The prepared extract has obvious phytoestrogen-like effect and is suitable for improving women's perimenopausal syndrome. The invention also relates to the application of the extract in the preparation of pharmaceutical preparations for treating perimenopausal syndrome and health food. Background technique [0002] With the increasing aging of the social population, the quality of life of the elderly has been widely valued by the society. The average life expectancy of women has increased year by year, which means that they will spend at least one-third of their time in perimenopause and postmenopause. Pass. Perimenopause refers to the peri...

Claims

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Application Information

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IPC IPC(8): A61K36/9068A61P15/12A61P5/30A23L1/29A23L33/00
Inventor 王发善韩振远陈玉忠褚弘斌刘卫东蹇小兵陈冬蕾
Owner DUODUO PHARMA
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