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SNP (Single Nucleotide Polymorphism) locus related to maize plant high character

A high-character, corn technology, applied in the field of alleles, can solve the problems of difficult production, excessive influence of agronomic characters, etc., and achieve the effect of speeding up the breeding process and broad application prospects.

Active Publication Date: 2012-03-14
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The adoption of dwarf varieties has greatly improved the yield of rice, wheat and other crops, triggering the first green revolution; and the plant height genes currently cloned in maize are mostly from mutants with extreme phenotypes. It has too much influence on other agronomic traits, so it is difficult to be widely used in production

Method used

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  • SNP (Single Nucleotide Polymorphism) locus related to maize plant high character
  • SNP (Single Nucleotide Polymorphism) locus related to maize plant high character
  • SNP (Single Nucleotide Polymorphism) locus related to maize plant high character

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1. Discovery and functional research of functional site SNP5259

[0034] 1. QTL-qPH1 fine mapping and cloning

[0035] (1) Construction and evaluation of QTL-qPH1 near-isogenic lines:

[0036] A near-isogenic line of the target gene QTL-qPH1 was constructed using a high-generation backcross strategy, such as figure 1 As shown, two families with extreme plant heights RIL88 (average plant height 136cm) and RIL279 (average plant height 236cm) in the recombinant inbred line population developed from the strong dominant combination Zong 3 and 87-1 were selected. segment, RIL88 carried a fragment from Zong3, while RIL279 carried a fragment from 87-1. The results of scanning 101 SSR markers in the whole genome showed that there were only 40% genetic differences between the two lines. A near-isogenic line (NIL 88\279 ).

[0037] In the early generations BC 1 f 1 , BC 2 f 1 and BC 3 f 1 Selection is based on phenotype only, and individuals with high plant heigh...

Embodiment 2

[0120] The development and application of the molecular marker of embodiment 2, PCR

[0121] In order to facilitate the better application of this rare allele in maize breeding and variety improvement, two flanking markers (public marker umc2396 and INDEL marker MH412) closely linked to it were screened and developed on both sides of SNP5259. An AC-PCR marker MN3 that directly detects this rare SNP was developed.

[0122] 1. The experimental materials are as follows:

[0123] (1). Five inbred lines with rare SNP5259(T): 81162, Ye107 (Ye107), Zong3 (Zong3) and W138,

[0124] (2). Ten inbred lines without rare SNP5259 (that is, the SNP here is G): 87-1, Zong31 (Zong31), Zheng58 (Zheng58), Chang7-2 (Chang7-2), B73 , Mo17, 4F1, Zheng 32 (Zheng32), By804, A619.

[0125] (3). Two parental materials used for fine positioning: RIL88 and NIL 88\279 .

[0126] 2. Primers for genotype detection:

[0127] Group 1: Primer MH412

[0128] MH412_F: SEQ ID NO:6; MH412_R: SEQ ID NO:7.

...

Embodiment 3

[0147] Example 3, qPH1 functional verification and effect evaluation

[0148] 1. The effect of qPH1 in different genetic backgrounds

[0149] In all scanned inbred lines, the functional site SNP (5259) of qPH1 shared two alleles, namely G(NIL 88\279 Genotype, expressed as qPH1+ / +) and T (RIL88 genotype, expressed as qPH1- / -). Because it can moderately reduce plant height, the Br2 allele in RIL88 is an excellent allele suitable for breeding. Since the target SNP is a rare SNP, the frequency in the natural population is too low to verify the function by association analysis. RIL88 and three inbred lines Zong3, Ye107 and 81162 containing this rare favorable SNP were selected and crossed with four conventional inbred lines with normal plant height respectively, and four F2 segregating populations were developed to increase the rare allele frequency to assess its effect. 4 hybrids were combined 81162×4F1, Ye 107×Zheng 32, Ye 107×B73 and Zong 3×Chuan 48-2 in Sanya, Hainan in the...

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Abstract

The invention discloses an SNP (Single Nucleotide Polymorphism) locus related to a maize plant high character, which is the nucleotide 5248 from the 5' tail end in SEQ ID NO:1, the nucleotide 5248 from the 5' tail end in SEQ ID NO:1 is positioned in the 5th exon of a maize dwarf mutant gene br2 in a maize genome, and the nucleotide 5248 is T. Molecular markers of the invention can be used for maize breeding in different genetic backgrounds, can be used for the genetic improvement of maize; and the method and the marks have wide application prospects in the field of maize breeding.

Description

technical field [0001] The invention relates to two alleles of a maize plant height gene Br2, their internal functional sites, encoded proteins and their application in crop genetics and breeding. Background technique [0002] Corn is not only an important food crop, but also an important feed source and industrial raw material. At the same time, as a C4 crop, corn is also an important energy crop. As the area of ​​cultivated land continues to decrease, increasing the yield per unit area is the primary choice to increase the yield of corn, while increasing the planting density per unit area is the most important way to increase the yield per unit area. Therefore, improving the plant type of maize to increase the planting density is an important direction of maize genetics and breeding, and the moderate reduction of plant height plays a vital role in regulating the plant type. The adoption of dwarf varieties has greatly improved the yield of rice, wheat and other crops, trig...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11A01H1/04
Inventor 李建生高玉峰严建兵邢安琪张卫平郑艳萍杨小红
Owner CHINA AGRI UNIV
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