Method by utilizing near infrared spectrum to measure content of multiple ingredients of complex sophora flavescens injection during percolation process

A technology of near-infrared spectroscopy and Sophora flavescens injection, which is applied in the field of quality control of traditional Chinese medicine production, can solve the problems of not meeting the requirements of rapid real-time monitoring, hindering the industrialization development of traditional Chinese medicine, low accuracy, etc., and achieves shortening analysis and measurement time and linear relationship. Good and accurate results

Active Publication Date: 2012-03-21
SHANXI ZHENDONG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods are cumbersome, time-consuming, and inaccurate, and are far from meeting the requirements for rapid and real-time monitoring of various components in the production process of Sophora flavescens preparations, which seriously hinders the industrialization of traditional Chinese medicine.

Method used

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  • Method by utilizing near infrared spectrum to measure content of multiple ingredients of complex sophora flavescens injection during percolation process
  • Method by utilizing near infrared spectrum to measure content of multiple ingredients of complex sophora flavescens injection during percolation process
  • Method by utilizing near infrared spectrum to measure content of multiple ingredients of complex sophora flavescens injection during percolation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Collection and preprocessing of calibration sample sets.

[0041] Take different batches of Sophora flavescens and Poria cocos medicinal materials, grind them into coarse powders of 0.2mm-0.81mm, weigh 70g of Sophora flavescens powder and 30g of Poria cocos medicine powder, mix them, add 1200ml of 1% acetic acid aqueous solution, soak for 24 hours, and pack the powder into Enter the percolation device, compact and start percolation. The filtrate is 600ml of 1% acetic acid aqueous solution, the percolation time is 24h, and the flow rate is 0.5ml / min. During the percolation process, samples were taken every 15 minutes to ensure that the content distribution of each component of the sample had a certain range, and a total of 80 samples were collected. The samples collected above were first filtered with four layers of filter paper, then centrifuged at 1000rmp for 10min, and the supernatant was taken for content determination and spectrum collection.

[0042] 2. Calibr...

Embodiment 2

[0065] The steps of the method are the same as those in Example 1, except that a calibration model for oxysophocarpine is established.

[0066] 1. The collection and pretreatment of the calibration sample set are the same as in Example 1.

[0067] 2. The determination of the content of oxysophocarpine in the calibration sample set is the same as in Example 1.

[0068] The measured distribution range of oxysophocarpine content in the calibration sample set is 0.026-0.522 mg / ml.

[0069] 3. The NIR spectrum data collection of the calibration sample set is the same as in Example 1.

[0070] 4. The establishment of the oxidized sophocarpine calibration model is the same as in Example 1.

[0071] Select SNV Pretreatment, 5388-6572cm -1 The performance of the PLS regression model established by Band is the best, that is, the maximum correlation coefficient is 0.980, the minimum RMSECV is 0.327 mg / ml, and the optimal number of factors is 3. The correlation between the reference v...

Embodiment 3

[0078] The steps of the method are the same as those in Example 1, except for the determination of the content of pterostaloside and the establishment of the calibration model.

[0079] 1. The collection and pretreatment of the calibration sample set are the same as in Example 1.

[0080] 2. Calibrate the determination method of the content of pteroside in the sample set.

[0081] HPLC chromatographic conditions: Phenomenex Luna C18 chromatographic column (250mm×4.6mm, 5um); mobile phase: acetonitrile:water=30:70; flow rate: 1.0mL / min; detection wavelength 310nm; column temperature 35°C.

[0082] The distribution range of the content of trilobalin in the calibration sample set was 0.002-0.041 mg / ml.

[0083] 3. The NIR spectrum data collection of the calibration sample set is the same as in Example 1.

[0084] 4. The establishment of the pterostaloside calibration model is the same as in Example 1.

[0085] Select SNV Pretreatment, 5388-6572cm -1 The performance of the PLS...

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Abstract

The invention relates to amethod by utilizing a near infrared spectrum to measure content of multiple ingredients of complex sophora flavescens injection during a percolation process, which comprises the following steps that: sophora flavescen and rhizoma heterosmilacis japonicae medicinal percolation liquid of different bathe is firstly collected as a correction sample set, content of each ingredient in the percolation liquid is measured as a reference value through a traditional method, the sample is scanned through a collecting way and collecting parameters to obtain a corresponding near infrared spectrum graph, and a multi-element correction model between the percolation liquid spectrum and the content of the ingredients is established through a chemometrics technology. When the percolation liquid with the content of the ingredients being unknown is detected, a near infrared spectrum of the percolation liquid is measured through the same way, and the content of each ingredient can be quickly obtained through the established correction model. The method is simple and quick to operate, has high accuracy and can effectively solve the quick detection of the content of multiple ingredients of the complex sophora flavescens injection during the percolation process, so the sophora flavescens preparation production is safely and effectively guaranteed.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine production quality control, and relates to a method for measuring component content by near-infrared spectroscopy, in particular to a method for quickly determining the content of various components in the percolation process of compound Sophora flavescens injection based on near-infrared spectroscopy. Background technique [0002] Compound Kushen Injection is a pure traditional Chinese medicine preparation refined from Sophora flavescens and Poria cocos. It has anti-cancer, immune-enhancing, hemostatic, pain-relieving and anti-tumor effects. Its main components are matrine, sophoridine and oxypicrin. Alkaloids such as ginseng. The ministerial standard uses the acid-base titration method to determine the total alkaloid content in the compound Sophora flavescens injection, but the current determination methods for the content of alkaloids in Sophora flavescens and its preparati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/35G01N21/3577G01N21/359
Inventor 瞿海斌程翼宇黄红霞陈晨王旭文李安平郝润才菅晓勇
Owner SHANXI ZHENDONG PHARMA
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