Endophyte bio-transformed raisin extract and preparation and application in flavoring thereof

Abstract

The present invention belongs to the technical field of perfume for tobacco, and particularly relates to endophyte bio-transformed raisin extract and preparation and application in flavoring thereof. The method is implemented as follows: (1) separating and purifying endophyte; (2) culturing endophyte; (3) extracting and refining raisin; and (4) bio-transforming raisin extract with the endophyte to obtain bio-transformed raisin extract. The method of the present invention has simple technological process and low cost, and the obtained bio-transformed raisin extract can be directly used for theflavoring and casing of cigarettes. The perfume of bio-transformed raisin extract has a unique fresh and sweet smell, and can lower stimulation after being added to cigarettes. The perfume of bio-transformed raisin extract has a strong fresh and sweet smell as well as a strong toasting smell.

Description

technical field

[0001] The invention relates to the technical field of biological preparation of tobacco spices, in particular to a plant endophyte biotransformation raisin extract and its preparation and application method in tobacco flavoring. Background technique

[0002] At present, there are two manufacturing methods of flavorings for tobacco, extracting natural flavorings from tobacco or other plants, or using traditional chemical methods to synthesize flavorings. Yet these two kinds of methods all have its respective limitation, for a certain plant spice, its aroma component and characteristic are constant, we are difficult to make the plant produce the spice with another kind of local flavor according to the needs of fragrance, and The development of plant-derived flavors is limited due to the limited available plant resources. For chemically synthesized fragrances, in addition to the single aroma, due to the limitation of synthesis methods and conditions, many exce...

Examples

Embodiment 1

[0023] (1) Isolation and purification of camptothecin endophytes

[0024] After scraping off the outer skin of the camptothecin bark with a sterile knife, rinse it with sterile water, cut it into small pieces of 1cm×1cm, put it in 75% ethanol for 1min, then sterilize it with 2.5% sodium hypochlorite solution for 10min, and then use Sterilize with 75% ethanol for 1 min, rinse with sterile water 2-3 times, cut off the edge, and insert it on the PDA plate medium. After mycelium grows on the surface of the bark, pick the tip part and move it to fresh PDA medium, repeat the separation and purification culture according to the plate streak separation method, and use an inoculation loop to dip a little bark in an aseptic operation to indicate the growth. Mycelium, continuously streaked on the surface of the sterile plate, the number of mycelial cells will decrease with the increase of the number of streaks, and gradually disperse, the microorganisms can be dispersed one by one, after...

technical field

[0001] The invention relates to the technical field of biological preparation of tobacco spices, in particular to a plant endophyte biotransformation raisin extract and its preparation and application method in tobacco flavoring. Background technique

[0002] At present, there are two manufacturing methods of flavorings for tobacco, extracting natural flavorings from tobacco or other plants, or using traditional chemical methods to synthesize flavorings. Yet these two kinds of methods all have its respective limitation, for a certain plant spice, its aroma component and characteristic are constant, we are difficult to make the plant produce the spice with another kind of local flavor according to the needs of fragrance, and The development of plant-derived flavors is limited due to the limited available plant resources. For chemically synthesized fragrances, in addition to the single aroma, due to the limitation of synthesis methods and conditions, many exce...

Examples

Embodiment 1

[0023] (1) Isolation and purification of camptothecin endophytes

[0024] After scraping off the outer skin of the camptothecin bark with a sterile knife, rinse it with sterile water, cut it into small pieces of 1cm×1cm, put it in 75% ethanol for 1min, then sterilize it with 2.5% sodium hypochlorite solution for 10min, and then use Sterilize with 75% ethanol for 1 min, rinse with sterile water 2-3 times, cut off the edge, and insert it on the PDA plate medium. After mycelium grows on the surface of the bark, pick the tip part and move it to fresh PDA medium, repeat the separation and purification culture according to the plate streak separation method, and use an inoculation loop to dip a little bark in an aseptic operation to indicate the growth. Mycelium, continuously streaked on the surface of the sterile plate, the number of mycelial cells will decrease with the increase of the number of streaks, and gradually disperse, the microorganisms can be dispersed one by one, after...

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