Earthworm antimicrobial peptide biological pesticide and application

A technology of biological pesticides and antimicrobial peptides, applied in the fields of application, biocide, chemicals for biological control, etc., can solve the problems such as no reports on earthworm antimicrobial peptide pesticides for preventing and controlling diseases and insect pests, and achieve the effect of simple production process

Inactive Publication Date: 2012-04-04
龚钢明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on earthworm antimicrobial peptide pesticides and its use in spraying

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Preparation of earthworm antimicrobial peptide biopesticide: Take 1000g of live earthworms and soak them in water for 2 hours, wash away the sundries with water, then rinse them with distilled water for 3 times, put them into a sink with positive and negative electrodes connected to the controller, add 2000mL phosphate buffer solution (pH5.8), the liquid level of the buffer solution is higher than the electrode, turn on the power supply, and under the voltage of 20V, electrically stimulate the earthworms for 0.5min, the stimulated earthworms discharge secretions into the phosphate buffer solution, Take out the earthworms, mince them with a tissue homogenizer, add 8000mL phosphate buffer (pH5.8), gently stir and extract for 3h, then heat to 80°C, keep warm for 15min, after cooling, centrifuge at 4000r / m for 15min , to obtain the centrifugate for later use, and take out the buffer solution in the water tank to merge with the above centrifugate, filter with a filter membran...

Embodiment 2

[0018] Preparation of earthworm antimicrobial peptide biopesticide: Take 1000g of live earthworms and soak them in water for 3 hours, wash away the sundries with water, rinse them with distilled water for 3 times, put them into a water tank with positive and negative electrodes connected to the controller, add 2000mL phosphate buffer solution (pH5.8), the liquid level of the buffer solution is higher than the electrode, turn on the power supply, and under the voltage of 15V, electrically stimulate the earthworms for 1.0min, the stimulated earthworms discharge secretions into the phosphate buffer solution, Take out the earthworms, mince them with a tissue homogenizer, add 7000mL phosphate buffer (pH5.8), gently stir and extract for 4h, then heat to 80°C, keep warm for 15min, after cooling, centrifuge at 4000r / m for 15min , to obtain the centrifugate for later use, and take out the buffer solution in the water tank to merge with the above centrifugate, filter with a filter membra...

Embodiment 3

[0021] Preparation of earthworm antimicrobial peptide biopesticide: Take 1000g of live earthworms and soak them in water for 4 hours, wash the sundries with water, rinse them with distilled water for 3 times, put them into a water tank with positive and negative electrodes connected to the controller, add 2000mL phosphate buffer solution (pH5.8), the liquid level of the buffer solution is higher than the electrode, turn on the power supply, and under the voltage of 15V, electrically stimulate the earthworms for 1.5min, the stimulated earthworms discharge secretions into the phosphate buffer solution, Take out the earthworms, mince them with a tissue homogenizer, add 6000mL phosphate buffer (pH5.8), gently stir and extract for 4h, then heat to 80°C, keep warm for 15min, after cooling, centrifuge at 4000r / m for 15min , to obtain the centrifugate for later use, and take out the buffer solution in the water tank to merge with the above centrifugate, filter with a filter membrane of...

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PUM

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Abstract

The invention provides an earthworm antimicrobial peptide biological pesticide and application and belongs to the field of biochemistry. A technical scheme is as below: extracting earthworm antimicrobial peptide from earthworm body cavity and tissue homogenate by a phosphate buffer; centrifuging a leaching liquor; carrying out ultrafiltration; and concentrating to prepare the earthworm antimicrobial peptide biological pesticide. The biological pesticide of the invention can be effectively applied to control of insect diseases of paddy rice, fruit and vegetable; and the production technology is simple and without generating pollution. The biological pesticide of the invention belongs to a green environment friendly product without pollution, and is especially suitable for production of nuisance-free organic agricultural products.

Description

technical field [0001] The invention relates to an earthworm antimicrobial peptide biological pesticide and its application in the prevention and control of crop diseases and insect pests. It specifically relates to the preparation of an earthworm antimicrobial peptide biopesticide and its application in the prevention and control of diseases and insect pests in the production of rice, fruits and vegetables. It belongs to the field of biochemical industry. Background technique [0002] The annual planting area of ​​rice, the main food crop in my country, exceeds 28 million hectares, and the planting area of ​​vegetables and fruits exceeds 1800 hectares. Among them, the area of ​​pest control is about 6.7 billion mu. Every year, the occurrence of pests and diseases will lead to a large area of ​​production reduction and quality reduction of these commercial crops. [0003] The current main control technology still relies on chemical pesticides. Extensive use of chemical pe...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P3/00A01P7/04
Inventor 龚钢明王忠豪
Owner 龚钢明
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