Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Gastric cancer cell line for expressing green fluorescent protein and luciferase and application thereof in cancer model

A technology of green fluorescent protein and luciferase, applied in the field of biomedical research, can solve the problems of inconvenient detection, low spatial resolution, and the need for substrates

Inactive Publication Date: 2012-04-04
FOURTH MILITARY MEDICAL UNIVERSITY
View PDF0 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, GFP also has its disadvantages: ① requires excitation light
The disadvantage is that the detection is inconvenient and requires a substrate; and due to the low spatial resolution and the limitation of the detection method, the morphology of the cells cannot be observed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gastric cancer cell line for expressing green fluorescent protein and luciferase and application thereof in cancer model
  • Gastric cancer cell line for expressing green fluorescent protein and luciferase and application thereof in cancer model
  • Gastric cancer cell line for expressing green fluorescent protein and luciferase and application thereof in cancer model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0046] Gastric cancer cell line expressing green fluorescent protein and luciferase, using gastric cancer SGC7901 as the mother cell, using the method of lentivirus infection, the green fluorescent protein GFP and firefly luciferase Firefly luciferase, LUC genes were brought into the cells to obtain stable expression of green fluorescence Protein and firefly luciferase cell lines, and different gastric cancer animal models were established by subcutaneous, tail vein and orthotopic injection of the cell lines.

[0047] The method of described lentiviral infection is to introduce foreign gene, and its steps are:

[0048] a) Lentiviral packaging plasmids pWPT-GFP, pMD2.G, pSPAX2, pLenti-CMV-puro-LUC, VSVg, pCMV-dR8.91 were amplified in Escherichia coli, identified and purified by enzyme digestion;

[0049] b) Co-transfect the plasmid obtained above into 293T cells by the liposome method according to a certain ratio, so as to generate a supernatant containing lentivirus;

[0050]...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of biomedical research, in particular to a gastric cancer cell line for expressing green fluorescent protein and luciferase, which is established by introducing the green fluorescent protein and the luciferase into a cell, and an application thereof in a cancer model. The gastric cancer cell line is characterized in that the gastric cancer cell line for stably expressing green fluorescent protein and luciferase is obtained by using a human gastric cancer cell line as mother cell and bringing the green fluorescent protein (GFP) and firefly luciferase (Fireflyluciferase, LUC) genes into the cell by using a slow virus infection method; and different gastric cancer models are established for the cell line by using hypodermic injection, tail vein injection and in situ injection methods. The invention provides establishment and application of the human gastric cancer cell line; and the gastric cancer cell line for expressing green fluorescent protein and luciferase is established by using the slow virus infection method, applied to the cancer model and used for gastric cancer preclinical studies.

Description

technical field [0001] The invention relates to the field of biomedical research, in particular to introducing green fluorescent protein and firefly luciferase into cells, establishing a gastric cancer cell line expressing green fluorescent protein and luciferase and applying it to a cancer model for basic and preclinical research on gastric cancer. Background technique [0002] According to the statistics of the World Health Organization in 2008, gastric cancer is the second most common malignant tumor after lung cancer. my country is a high-incidence area of ​​gastric cancer. According to statistics, there are more than 400,000 new cases of gastric cancer each year, and nearly 300,000 deaths, ranking first in the world. According to the second national cause of death survey conducted by the Ministry of Health from 1990 to 1992, gastric cancer accounted for about a quarter of all cancer deaths. Gastric cancer has increasingly become a major disease affecting people's healt...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/10C12N15/867A01K67/027A61K49/00
Inventor 吴开春胡皓聂勇战殷继鹏
Owner FOURTH MILITARY MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com