Method for detecting small organic biological molecules with graphene electrode-based molecular device

Abstract

The invention discloses a method for detecting small organic biological molecules and a special graphene electrode-based DNA (deoxyribonucleic acid) unimolecular connecting device. The DNA unimolecular connecting device is prepared by the following method: 1) constructing a molecular device with graphene electrodes; 2) treating the grapheme molecular device obtained in step 1) so as to obtain nano-gaps of 1-10nm; 3) connecting a designed DNA sequence to the nano-gaps of a cutting device, thus obtaining a DNA unimolecular connecting device. And detection method consists of: using the prepared DNA unimolecular connecting device to combine with and identify small organic biological molecules for electric signal detection, excluding various interference factors; subjecting the small organic biological molecules to sensitivity and other tests in the device; determining a measuring condition according to the test results of the above steps so as to realize detection of the small organic biological molecules in the connecting device. The method of the invention makes use of a functionalized unimolecular device to realize high sensitivity detection of small organic biological molecules through electric signals.

Description

technical field [0001] The invention relates to a method for detecting organic biological small molecules by a graphene electrode-based molecular device. Background technique [0002] Single-molecule detection is a hot spot in scientific research today. Single-molecule detection, as an analysis method for the properties and behaviors of limited and countable microscopic individuals, can provide microscopic individual information that cannot be obtained by traditional macroscopic analysis methods. , clinical medical diagnosis and so on have more important significance. For the research methods of detecting organic biological small molecules, surface plasmon resonance, fluorescence spectroscopy, electrochemistry, scanning probe microscopy, etc. have been developed. Some existing methods lack higher sensitivity and are greatly affected by environmental factors. It is urgent to establish a system for rapid and highly sensitive detection of a single biochemical event. (1: Liu, ...

AI Technical Summary

Problems solved by technology

Some existing methods lack higher sensitivity and are greatly affected by environmental factors. It is urgent to establish a system for rapid and highly sensitive detection of a single biochemical event.

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