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Mycobacterium tuberculosis drug-resistant mutant gene detection kit

A technology for Mycobacterium tuberculosis and drug resistance mutation, applied in the field of genes, can solve the problems of time-consuming and labor-intensive, unable to achieve rapid clinical detection, and achieve the effect of increasing accuracy

Active Publication Date: 2013-05-01
GUANGDONG HYBRIBIO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Hybridization methods can improve the specificity of DNA analysis, but most hybridization methods are time-consuming and labor-intensive, and cannot meet the requirements of clinical rapid detection

Method used

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  • Mycobacterium tuberculosis drug-resistant mutant gene detection kit
  • Mycobacterium tuberculosis drug-resistant mutant gene detection kit
  • Mycobacterium tuberculosis drug-resistant mutant gene detection kit

Examples

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Embodiment Construction

[0084] The present invention will be described in detail below through examples.

[0085] In the examples, 20% EDAC solution, 0.1% SDS, 0.25% skimmed milk powder, 0.05% thimerosal, and 0.05% sodium azide all refer to the mass ratio, and 0.1% Tween 20 refers to the volume ratio.

[0086] Step 1 Preparation of gene chip

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Abstract

The invention discloses a mycobacterium tuberculosis drug-resistant mutant gene detection kit comprising: (1) a gene chip which has (i) a nucleotide probe, wherein the probe is a sequence of SEQIDNos: 1-31 or a complementary sequence of SEQIDNos: 1-31, (ii) a DNA sequence labeled with biotin points, wherein the sequence is SEQIDNO. 32, (iii) an IS6110DNA sequence of a mycobacterium tuberculosis complex, wherein the sequence is SEQIDNo. 33; (2) various primers used for amplifying DNA sequences in clinical samples, wherein the DNA sequences of the primers are SEQIDNos. 34-41. The kit of the invention detects the drug resistance of mycobacterium tuberculosis to rifampin and isoniazid, provides reference basis for the prevention and treatment of tuberculosis, and has significant meaning.

Description

technical field [0001] The invention relates to the field of genes, in particular to a detection kit for the mutation gene of Mycobacterium tuberculosis (MTB) drug resistance (rifampicin and isoniazid). Background technique [0002] Tuberculosis is a highly contagious chronic wasting disease caused by Mycobacterium tuberculosis (MTB) invading the human body. It has a high incidence and is extremely harmful. WHO has regarded tuberculosis as one of the key communicable diseases to be controlled, and has designated March 24th as "World Tuberculosis Day" since 1995 to remind the public to deepen their understanding of tuberculosis. [0003] According to WHO reports, there are 20 million tuberculosis patients in the world, and 8 million new cases are added every year, and about 3 million people die from tuberculosis every year, ranking first in the death toll of various infectious diseases. According to the latest survey by the Ministry of Health, 550 million people in my countr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/04C12R1/32
Inventor 何海华李烈军
Owner GUANGDONG HYBRIBIO BIOTECH CO LTD
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