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Microbe used for degrading deoxynivalenol

A technology of microorganisms and vomitoxin, applied in bacteria and other directions, can solve the problems of difficult operation, restrict wide application, reduce the nutritional quality and palatability of feed, etc., and achieve the effect of stable performance.

Active Publication Date: 2012-06-06
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current detoxification methods for feed mildew include: physical detoxification method, chemical detoxification method, and enzymatic hydrolysis method. Feed machine raw materials cannot be processed by this method, and the nutritional quality and palatability of the feed will often be reduced after chemical detoxification.
The enzymatic hydrolysis method has less loss and influence on the nutritional components of the feed, but its high cost and unstable effect restrict the wide application of this method
[0004] Microbial decomposition of vomitoxin has the following advantages: specific and effective, mild conditions: no harmful chemical substances are introduced, less loss of nutritional value of feed, no environmental pollution, and has attracted widespread attention. very little research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1. Screening and separation of microorganisms decomposing vomitoxin

[0052] (1) Weighing of animal intestinal contents

[0053] Aseptically remove the contents of goose intestines, mix the contents in an anaerobic incubator, weigh 0.15g into a 2ml sterile centrifuge tube, and store them in a -20°C refrigerator until use.

[0054] (2) Pressure screening

[0055] Inoculate the intestinal contents prepared in step (1) into 2ml of basal medium containing 100ppm DON, mix well, place in an anaerobic incubator, and culture anaerobically at 30°C for 4 days. DON and DOM content, and calculate the conversion rate of DOM / (DON+DOM)×100%, and select the samples with conversion rate above 90%.

[0056] Inoculate the above-mentioned culture with a DOM conversion rate of more than 90% into the basal medium containing 100ppm DON according to the inoculum size of 10%, mix well, place it in an anaerobic incubator, and culture it anaerobically at 30°C for 4 days. The content of...

Embodiment 2

[0071] Embodiment 2, strain identification

[0072] Bacteria identification is to study the morphological and physiological and biochemical characteristics of the selected strains with the method of microbial classification and identification, and at the same time combine a molecular-based identification method.

[0073] The bacterial classification that the present invention isolates, screens is Clostridium sp., and the biological characteristic of this bacterial classification is as follows: the colony shape is less than the round white small colony of 0.5mm, cell rod shape, 0.3~2.0 μ m×1.5~2.0 μm, often arranged in pairs or short chains, with rounded or acuminate ends, motile with perigenetic flagella. The spores are ellipsoid or spherical cysts enlarged. Can utilize glucose, cellobiose, etc., catalase negative, obligate anaerobic, growth temperature is 10°C-65°C.

Embodiment 3

[0074] Embodiment 3, the stability of a strain of deoxynivalenol degrading microorganism according to the present invention

[0075] The strain of the present invention has a stable performance in decomposing vomitoxin, and the efficiency of degrading vomitoxin is still 100% when subcultured in L10 medium for 12 generations.

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Abstract

The invention provides a microbe used for degrading deoxynivalenol, which belongs to microbe and feed additive fields. The microbe for degrading deoxynivalenol is obtained from the content in animal intestinal tract by pressure selection, gradient dilution and separation as well as screening, and is clostridium sp. by identification. The stability of the strain for degrading deoxynivalenol is good, in a L10 medium, the subcultring is carried out for 8-12 generations, the degradedation rate of DON is 100%. In the L10 medium, the initial concentration of DON is 25-100 ppm, the pH value is 5.0-8.0, anaerobic cultivation is carried out for 48-96 hours at the temperature of 30-40 DEG C, and the degradation rate of DON is more than 90%.

Description

technical field [0001] The invention relates to a microorganism capable of degrading vomitoxin by isolating and screening from animal intestinal contents, and belongs to the field of feeding microorganisms and feed additives. Background technique [0002] Vomitoxin (vomitoxin), also known as deoxynivalenol (deoxynivalenol, DON), is a toxic metabolite produced by Fusarium, etc., and belongs to the trichothecene compound. DON contamination of grains is very common. People and animals can produce a wide range of toxic effects after eating grains contaminated by the toxin. The clinical symptoms include: unsteady standing, unresponsiveness, piloerection, loss of appetite, vomiting, etc. Among them, pigs are the most sensitive to vomitoxin. [0003] The current detoxification methods for feed mildew include: physical detoxification method, chemical detoxification method, and enzymatic hydrolysis method. Feed machine raw materials cannot be processed by this method, and the nutri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 王金全蔡辉益孙占敏李红霞王春阳
Owner FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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