Preservation method for stock spawn of straw mushrooms

A technology for mother seed and straw mushroom, applied in the field of edible mushroom production, can solve the problems of short preservation period of straw mushroom strain, high protection cost, complicated method, etc., and achieve the effects of easy popularization, low cost, and low technical and equipment requirements.

Inactive Publication Date: 2012-06-13
HUNAN CHUNHUA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to invent a new method that is long-acting, simple, practical and has a good preservation effect in view of the problems of short preservation period, high protection cost and cumbersome methods of Strain mushroom strains.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The specific implementation steps of the mother species of straw mushroom preservation method are as follows:

[0020] Production of mother seed culture medium: (1) take by weighing 200 grams of peeled and sliced ​​potatoes, place them in a pot, add 1000ml of water, cook the potatoes until they are cooked and not rotten; (2) filter with gauze, and get the filtrate; ( 3) Add 20 grams of agar to the filtrate, continue to cook until the agar melts, then add 18 grams of glucose, and replenish water to 1000ml; (4) Fill the cooked medium to 1 / 5 of the length of the test tube while it is hot, and cover it with silica gel (5) Sterilize at 121°C for 30 minutes, place the test tube at an angle after the temperature of the test tube is lower than 70°C after sterilization, and spread the front part of the medium to 1 / 2 of the test tube.

[0021] Inoculation: Under sterile conditions, use an inoculation needle to take a certain size of the mother seed medium, move it into the middle...

Embodiment 2

[0025] The specific implementation steps of the mother species of straw mushroom preservation method are as follows:

[0026] Production of mother seed culture medium: (1) take 300 grams of peeled and sliced ​​potatoes, place them in a pot, add 1200ml of water, cook the potatoes until they are cooked and not rotten; (2) filter with gauze, and get the filtrate; ( 3) Add 15 grams of agar to the filtrate, continue to cook until the agar melts, then add 20 grams of glucose, and replenish water to 1200ml; (4) Fill the cooked medium to 1 / 5 of the length of the test tube while it is hot, and cover it with silica gel (5) Sterilize at 115°C for 50 minutes, place the test tube at an angle after the temperature is lower than 70°C after sterilization, and spread the front part of the medium to 1 / 2 of the test tube.

[0027] Inoculation: Under sterile conditions, use an inoculation needle to take a certain size of the mother seed medium, move it into the middle of the slant medium, sterili...

Embodiment 3

[0031] The specific implementation steps of the mother species of straw mushroom preservation method are as follows:

[0032] Production of mother seed culture medium: (1) take 150 grams of peeled and sliced ​​potatoes, place them in a pot, add 700ml of water, cook the potatoes until they are cooked and not rotten; (2) filter with gauze, and get the filtrate; ( 3) Add 10 grams of agar to the filtrate, continue to cook until the agar melts, then add 12 grams of glucose, and replenish water to 700ml; (4) Fill the cooked medium to 1 / 5 of the length of the test tube while it is hot, and cover it with silica gel (5) Sterilize at 127°C for 25 minutes, place the test tube at an angle after the temperature of the test tube is lower than 70°C after sterilization, and spread the front part of the medium to 1 / 2 of the test tube.

[0033]Inoculation: Under sterile conditions, use an inoculation needle to take a certain size of the mother seed medium, move it into the middle of the slant m...

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PUM

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Abstract

The invention discloses a preservation method for stock spawn of straw mushrooms. The method comprises the steps of preparation of a mother culture medium, inoculation, culture of activated stock spawn, protection of activated stock spawn, etc. According to the invention, a straw mushroom strain can be preserved for more than one year at a temperature of 1 to 4 DEG C; the method is simple to operate, has low requirements for technology and equipment, is easy to popularize and has a good preservation effect; a centrifuge tube is used to substitute a test tube for preservation of the strain, the volume of the centrifuge tube is small, and demands for transfer of tubes can be satisfied by only taking one centrifuge tube each time; stock spawn is used as a seed tuber, the seed tuber has a small volume, and a protective agent can fully wrap the seed tuber, stabilizes configuration of cell components through affinity to water and cells generated by oxygen and ionic bonds and protects hypha from being damaged by low temperature; the method is an effective alternative technology for conventional preservation methods for stock spawn of straw mushrooms.

Description

technical field [0001] The invention relates to the technical field of edible fungus production, in particular to a method for preserving mother species of straw mushrooms. Background technique [0002] Straw mushroom is also known as Baojiao mushroom, stalk mushroom, sesame mushroom, orchid mushroom. Its meat is crisp and tender, delicious, and has a special umami taste with chicken essence and monosodium glutamate. It is one of the favorite summer edible mushrooms. It can be called a good product. my country is the birthplace of straw mushroom cultivation. As early as the early Ming Dynasty, there were records of planting straw mushrooms in Shaoguang, Guangdong and Liuyang, Hunan. At present, more than 20 countries and regions in the world have carried out artificial cultivation. [0003] Straw mushroom is a high-temperature mushroom, which is native to tropical and subtropical regions with high temperature and rain, such as Guangdong, Fujian, Taiwan, Hainan, Guangxi, Hun...

Claims

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Application Information

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IPC IPC(8): C12N1/04
Inventor 王小艳王春晖彭运祥姜性坚胡汝晓李新菊
Owner HUNAN CHUNHUA BIOTECH
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