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Method for identifying soil pollution under assistance of springtail reproduction

A technology for soil pollution and auxiliary identification, applied in soil material testing, material inspection products, etc., can solve the problems of scientifically characterizing the overall quality characteristics of polluted soil, chronic toxicity hazards, high cost of chemical analysis, etc., and achieves wide application range and low price , the effect of high sensitivity

Inactive Publication Date: 2012-06-13
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, persistent organic pollutants (POPs), which people have paid attention to in recent years, mostly have "three causes" effects and bioaccumulation, and often exist in the soil environment for a long time in the form of compound pollution. The content is low, but its long-term chronic toxicity will cause great harm to the human body
The cost of traditional chemical analysis is high, and due to the limitations of instrumental methods, only part of the pollutants can be monitored, and in many cases, it is impossible to comprehensively and scientifically characterize the overall quality characteristics of contaminated soil

Method used

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  • Method for identifying soil pollution under assistance of springtail reproduction
  • Method for identifying soil pollution under assistance of springtail reproduction
  • Method for identifying soil pollution under assistance of springtail reproduction

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0034] Embodiment 1, using the method of the present invention to assist in the identification of soil pollution

[0035] The soil to be tested in this example is the soil collected from a coking plant in Beijing (the pollutants discharged from a coking plant are usually mainly 2-6-ring polycyclic aromatic hydrocarbons).

[0036] 1. Weigh 150g of the soil to be tested (or 150g of artificial standard soil), and divide it into five 100mL small beakers (each small beaker has 30g of soil), add an appropriate amount of deionized water to make the soil moisture content 60%.

[0037] 2. Add 10 same-age Baifutiao to each small beaker in step 1 (the age of the insect is 10-12 days), then seal the small beaker with a parafilm, and put it into an artificial climate box for cultivation (20°C; The dark ratio is 12h:12h, the light intensity is 400Lux; the air humidity is 70%) for 28 days; the following treatment is carried out every 7 days: remove the sealing film, remove the hyphae and imp...

Embodiment 2

[0043] Embodiment 2, using the method of the present invention to assist in the identification of soil pollution

[0044] The soil to be tested in this embodiment is the soil collected from farmland in the suburbs of Beijing.

[0045] 1. Weigh 150g of the soil to be tested (or 150g of artificial standard soil), and divide it into five 100mL small beakers (each small beaker has 30g of soil), add an appropriate amount of deionized water to make the soil moisture content 60%.

[0046] 2. Add 10 same-age Baifutiao to each small beaker in step 1 (the age of the insect is 10-12 days), then seal the small beaker with a parafilm, and put it into an artificial climate box for cultivation (20°C; The dark ratio is 12h:12h, the light intensity is 400Lux; the air humidity is 70%) for 28 days; the following treatment is carried out every 7 days: remove the sealing film, remove the hyphae and impurities on the soil surface with a toothpick, add about 1mL deionized water, supplemented with 2mg...

Embodiment 3

[0052] Embodiment 3, using the method of the present invention to assist in the identification of soil pollution

[0053] The preparation method of the soil to be tested in this embodiment is as follows: Pentachlorophenol is added to the artificial standard soil so that the final concentration is 200 mg / kg.

[0054] 1. Weigh 150g of the soil to be tested (or 150g of artificial standard soil), and divide it into five 100mL small beakers (each small beaker has 30g of soil), add an appropriate amount of deionized water to make the soil moisture content 60%.

[0055] 2. Add 10 same-age Baifutiao to each small beaker in step 1 (the age of the insect is 10-12 days), then seal the small beaker with a parafilm, and put it into an artificial climate box for cultivation (20°C; The dark ratio is 12h:12h, the light intensity is 400Lux; the air humidity is 70%) for 28 days; the following treatment is carried out every 7 days: remove the sealing film, remove the hyphae and impurities on the...

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Abstract

The invention discloses a method for identifying soil pollution under the assistance of springtail reproduction. The method comprises the following steps of: performing the following parallel operation on soil to be tested and artificial standard soil: (1) breeding springtails in the soil for 28 to 35 days, and (2) counting springtail larva, wherein if the number of the springtail larva in the soil to be tested is smaller than that of the springtail larva in the artificial standard soil, the soil to be tested is determined as candidate polluted soil. The method can be applied to soil polluted by various chemical substances, is wide in applicability range, is high in sensitivity compared with the conventional physical-chemical method, and is easy to operate and low in price.

Description

technical field [0001] The invention relates to the technical field of soil pollution monitoring and control, in particular to a method for assisting in identifying soil pollution, more specifically, using the number of larvae reproduced by collembola to assist in identifying soil pollution. Background technique [0002] Soil is an important element of the ecological environment. In recent years, with the continuous development of social economy, people's demand for various chemicals has increased day by day, resulting in many toxic and harmful chemicals entering the soil ecosystem during production and use, soil pollution The problem is becoming more and more prominent. Soil is an indispensable and important carrier of geochemical cycles, and its interaction with environmental elements such as water, atmosphere, and organisms leads to the cross-circulation of pollutants in various environmental carriers. As an irreplaceable natural resource for human survival, once soil is...

Claims

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Application Information

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IPC IPC(8): G01N33/24
Inventor 乔敏朱永官张偲王光鹏张轩
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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