Identification method of Tea plant germ plasma resource seedling-stage coldness resistance

An identification method and cold resistance technology, applied in horticultural methods, botanical equipment and methods, seed and rhizome treatment, etc., can solve the problems of relatively large natural environment restrictions and high cost of direct identification, and achieve fast, accurate and low-cost identification , the effect of easy operation

Inactive Publication Date: 2012-06-20
QINGDAO AGRI UNIV
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  • Claims
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Problems solved by technology

However, the cost of direct identification is relatively high, and it is greatly restricted by the natural environment.
In order to overcome this difficulty, people conduct indirect identification based on related indicators, but most of them only use a single indicator (such as plasma membrane permeability) for identification

Method used

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  • Identification method of Tea plant germ plasma resource seedling-stage coldness resistance
  • Identification method of Tea plant germ plasma resource seedling-stage coldness resistance
  • Identification method of Tea plant germ plasma resource seedling-stage coldness resistance

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Embodiment Construction

[0022] Through the specific examples given below, the present invention can be further understood, but they are not limitations of the present invention.

[0023] 1) From the "Huangshanzhong" tea garden introduced and planted in 1979 in Pingjia Village, Rizhao City, Shandong Province, the tea tree seeds that grew well and had not suffered frost damage were collected and mixed in the Rizhao Nursery of the Tea Germplasm Resource Nursery of Qingdao Agricultural University. From them, 28 tea tree seedlings with good growth were selected, and Pingyang Tezao and Zhenong 117 clones were used as controls.

[0024] 2) During the overwintering period of the tea tree, 3 leaves of the young tea tree were collected as a single plant for later use.

[0025] 3) The relative permeability of the plasma membrane of the leaves was measured by a conductivity meter, the soluble sugar content of the leaves was measured by anthrone sulfate colorimetry, and the soluble protein content of the leaves w...

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Abstract

The invention relates to an identification method of tea plant germ plasma resource seedling-stage coldness resistance, which comprises the following steps of: (1) culture of materials; (2) stress treatment of tea plant seedlings; (3) determination of physiological and biochemical indexes of tea plant leaves; (4) processing of experimental data; and (5) evaluation of coldness resistance. The method is simple and convenient to operate, is low in cost, and is fast and accurate to identify. Plasmalemma relative permeability of leaves can reflect the damage degree of the cell membrane; and soluble sugar and soluble protein are important permeability adjusting substances for the tea plant leaves, so the coldness resistance of the tea plant is stronger when the content of the soluble sugar and the content of the soluble protein are higher. The method is simple, convenient and feasible to evaluate the coldness resistance of the tea plant.

Description

technical field [0001] The invention belongs to the technical field of early identification of tea tree germplasm resources, and specifically introduces a method for identifying the cold resistance of tea tree germplasm resources at seedling stage. Background technique [0002] Tea tree is native to tropical and subtropical regions, and is a kind of foliage plant that likes warm climate conditions. In recent years, on the one hand, with the increasing demand for tea, the planting area of ​​tea has been moving northward; on the other hand, with the instability of climate change, tea trees frequently encounter low temperature damage such as "frost" or "cold spring" during winter and early spring. , causing damage to the new shoots of the tea tree, affecting the yield and quality of the tea, and even causing the death of the whole plant in severe cases. Therefore, it is very important to improve the cold resistance of tea trees and make them survive the winter safely. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G7/06A01C1/00
Inventor 丁兆堂王玉范凯
Owner QINGDAO AGRI UNIV
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