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Novel high-efficient quick virus elimination technique for strawberries

A virus-free and new technology, applied in the field of efficient and rapid virus-free technology for strawberries, can solve problems such as deformity, hindering strawberry production and development, and excessive pesticide residues.

Inactive Publication Date: 2012-06-20
天津中植科生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The economic benefits of strawberries have dropped significantly, seriously hindering the development of strawberry production
At the same time, the strawberries produced are of poor quality, and the fruit is small and deformed; therefore, strawberry virus disease is an important disease that affects the yield and quality of strawberries, and currently no effective pesticides are used to prevent and control viral diseases, even if some pesticides can be used, but Viral diseases are poorly prevented and often cause pesticide residues to exceed the standard. At present, viral diseases have become the "cancer" of strawberry production

Method used

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  • Novel high-efficient quick virus elimination technique for strawberries
  • Novel high-efficient quick virus elimination technique for strawberries
  • Novel high-efficient quick virus elimination technique for strawberries

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Embodiment Construction

[0017] The technical process of this patent:

[0018] 1. Collect virus-infected material-buds of excellent strawberry varieties.

[0019] 2. Wash the strawberry sprouts and rinse with running water for 10 minutes.

[0020] 3. Carefully peel off 3-5 outer leaves and take out the central bud.

[0021] 4. Short-term pretreatment of virus-containing strawberry buds at a high temperature of 40-50°C for 5-20 minutes.

[0022] 5. Sterilize strawberry buds with 0.1% mercuric chloride and 2% sodium hypochlorite for 10 minutes.

[0023] 6. Place the strawberry buds in the aseptic state under a stereo microscope, carry out aseptic dissection, and take out the meristematic tissue of 0.2-0.5mm, that is, the growth point.

[0024] 7. Under sterile conditions, place the growing point on the optimized cell initiation medium and culture it for 40 days to obtain virus-free callus.

[0025] 8. Under sterile conditions, the virus-free callus was transferred to the best strawberry propagation ...

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Abstract

The invention relates to a novel high-efficient quick virus elimination technique for strawberries, which comprises four aspects that: firstly a high-temperature short-time inactivation virus technique is adopted to perform short-time pretreatment about 5 to 20 minutes at the high temperature of 40 to 50 DEG C to realize an effect that partial virus is killed or the virus is inactivated, then the size of meristematic tissues for the best virus elimination effect is determined as 0.2 to 0.5mm under a stereoscopic vision microscope through a meristematic tissue size optimization technique, mitosis and growth of virus-free cell clusters are started through a cell starting culture medium formula, a series of optimization tests for the culture medium are carried out through mass Caespitose shoots culture technique to obtain the best strawberry breeding culture medium formula, and achieved annual production of one million of virus-eliminated strawberries seedlings technical level. By adopting the technique, the virus elimination effect can reach 90 to 100 percent, the virus-eliminated strawberry seedlings are applied and experimented on the production for ten years, the quantity of the application trial points reaches more than 100, and the yield increase effect reaches about 50 percent.

Description

technical field [0001] The invention relates to the field of new agricultural biotechnologies, in particular to a new technology for fast and efficient virus removal from strawberries. Background technique [0002] my country has developed rapidly in recent years. However, strawberries are easily infected with a variety of viral diseases. Strawberry infected with viral diseases has shrunken leaves and slow growth. The fruit is getting smaller and deformed year by year, and the quality is poor. Generally, the yield is reduced by 30-80%. . and increased year by year. The economic benefits of strawberries have dropped significantly, seriously hindering the development of strawberry production. At the same time, the strawberries produced are of poor quality, and the fruit is small and deformed; therefore, strawberry virus disease is an important disease that affects the yield and quality of strawberries, and currently no effective pesticides are used to prevent and control vira...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 高山林
Owner 天津中植科生物技术有限公司
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