Novel high-efficient quick virus elimination technique for strawberries
A virus-free and new technology, applied in the field of efficient and rapid virus-free technology for strawberries, can solve problems such as deformity, hindering strawberry production and development, and excessive pesticide residues.
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[0017] The technical process of this patent:
[0018] 1. Collect virus-infected material-buds of excellent strawberry varieties.
[0019] 2. Wash the strawberry sprouts and rinse with running water for 10 minutes.
[0020] 3. Carefully peel off 3-5 outer leaves and take out the central bud.
[0021] 4. Short-term pretreatment of virus-containing strawberry buds at a high temperature of 40-50°C for 5-20 minutes.
[0022] 5. Sterilize strawberry buds with 0.1% mercuric chloride and 2% sodium hypochlorite for 10 minutes.
[0023] 6. Place the strawberry buds in the aseptic state under a stereo microscope, carry out aseptic dissection, and take out the meristematic tissue of 0.2-0.5mm, that is, the growth point.
[0024] 7. Under sterile conditions, place the growing point on the optimized cell initiation medium and culture it for 40 days to obtain virus-free callus.
[0025] 8. Under sterile conditions, the virus-free callus was transferred to the best strawberry propagation ...
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