Method for high-efficiently realizing separation of soil animals and soil in crude products obtained by Tullgren method

Abstract

A method for high-efficiently realizing separation of soil animals and soil in crude products obtained by a Tullgren method relates to a separation method and resolves problems that extraction efficiency of soil animals in crude products obtained by the Tullgren method is low, and the individually small, soil animals with the color similar to that of the soil are not easy to be found. The method includes preparing sucrose solution; adopting the Tullgren method to separate the soil animals, pouring liquid supernatant and collecting the soil animals after a beaker for collecting the soil animals is in standing, adding the sucrose solution into the crude products in the beaker, standing the beaker after being oscillated, pouring liquid into a culture dish and repeatedly using the sucrose solution to realize separation; and taking large-size soil animals, cleaning the large-size soil animals by alcohol, collecting and storing the large-size soil animals, then sucking medium and small-size soil animals, cleaning the medium and small-size animals by alcohol, and collecting and storing the medium and small-size animals after the medium and small-size animals are repeatedly cleaned, so that the steps of the method are finished. The method resolves the problem that the individually small animals with the color similar to that of the soil are not easy to be found, separation efficiency is improved by once to ten times, and statistical accuracy is improved by 5% to 10%.

Description

technical field

[0001] The present invention relates to a method of separation. Background technique

[0002] Soil fauna refers to animals that live in soil or surface litter layers. Soil animals play an important role in decomposing biological residues, changing soil physical and chemical properties, promoting soil material cycle and energy conversion. To study soil animals, they need to be isolated from wild soil. The Tullgren method is a commonly used method for separating and collecting soil animals. It is to put the collected soil on a sieve with an aperture of 2mm, cover and bake it with a tube cover equipped with a 25-40W incandescent lamp above the sieve, and put it under the sieve. One funnel, put a 50mL beaker with 10-20mL alcohol under the outlet of the funnel. After the light is turned on, the temperature of the soil sample in the separator rises and the moisture decreases. The soil animals are heated and move down until they leave the soil and fall into the a...