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Biomolecular functionalized graphene/gold nano-particle composite film and preparation method thereof

A technology of gold nanoparticles and composite films, which is applied in the field of biomolecular functionalized graphene/gold nanoparticles composite films and its preparation, can solve the problems of difficult control and limited application, and achieve low detection limit and good reproducibility , detect the effect of signal sensitivity

Inactive Publication Date: 2013-10-16
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since this method utilizes the spontaneous reduction of chloroauric acid ions during the assembly process, the size distribution of the synthesized gold nanoparticles is wide from 0 to 200 nm, which is difficult to control and limits the application in catalytic and sensor devices.

Method used

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  • Biomolecular functionalized graphene/gold nano-particle composite film and preparation method thereof
  • Biomolecular functionalized graphene/gold nano-particle composite film and preparation method thereof
  • Biomolecular functionalized graphene/gold nano-particle composite film and preparation method thereof

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Embodiment 1

[0024] A preparation of bovine serum albumin functionalized graphene: the monolithic graphene oxide prepared from natural flake graphite is used as raw material to prepare a 0.01g / L graphene oxide aqueous dispersion, and 10mL graphene oxide aqueous dispersion is mixed with 1.25 Mix bovine serum albumin aqueous solution with a concentration of 0.8g / L, adjust the pH value of the mixed solution to 13 with 0.2mol / L NaOH aqueous solution, and react at 90°C for 2 hours; °C for 24 hours under vacuum to obtain BSA-G powder. Redisperse BSA-G powder in distilled water to obtain BSA-G with concentrations of 0.01g / L and 0.02g / L respectively - Aqueous solution; use 0.5mol / L hydrochloric acid aqueous solution to adjust the concentration of BSA-G to 0.02g / L - The pH value of the aqueous solution is 2, resulting in a concentration of 0.02g / LBSA-G + of aqueous solution.

[0025] Preparation of B gold nanoparticles: 50mL 1.0mmol / LHAuCL 4 The aqueous solution was heated to boiling, and 50 mL...

Embodiment 2

[0030] A preparation of bovine serum albumin functionalized graphene: the monolithic graphene oxide prepared from natural flake graphite is used as raw material to prepare a 5.0g / L graphene oxide aqueous dispersion, and 10mL graphene oxide aqueous dispersion is mixed with 50mL Mix bovine serum albumin aqueous solution with a concentration of 1.0g / L, adjust the pH value of the mixed solution to 11 with 2.0mol / L NaOH aqueous solution, and react at 80°C for 3 hours; centrifuge, wash with distilled water, remove the lower layer and precipitate at 50°C Dry under vacuum for 12 hours to obtain BSA-G powder. Redisperse the BSA-G powder in distilled water to obtain BSA-G with a concentration of 3.0g / L - 2 parts of aqueous solution; 1 part of BSA-G - The aqueous solution is adjusted to pH 2 with 3.0mol / L hydrochloric acid aqueous solution to obtain a concentration of 3.0g / LBSA-G + of aqueous solution.

[0031] Preparation of B gold nanoparticles: 50mL 2.0mmol / L HAuCL 4 Heat the aque...

Embodiment 3

[0036] A preparation of bovine serum albumin functionalized graphene: the monolithic graphene oxide prepared from natural flake graphite is used as raw material to prepare a 1.0g / L graphene oxide aqueous dispersion, and 10mL graphene oxide aqueous dispersion is mixed with 40mL Mix bovine serum albumin aqueous solution with a concentration of 0.5g / L, adjust the pH value of the mixed solution to 12 with 1.0mol / L NaOH aqueous solution, and react at 90°C for 1 hour; centrifuge, wash with distilled water, take the lower layer and precipitate at 50°C Dry under vacuum for 12 hours to obtain BSA-G powder. Redisperse the BSA-G powder in distilled water to obtain BSA-G with a concentration of 1.0g / L - 2 parts of aqueous solution; 1 part of BSA-G - The aqueous solution is adjusted to pH 3 with 0.1mol / L hydrochloric acid aqueous solution to obtain BSA-G with a concentration of 1.0g / L + of aqueous solution.

[0037] Preparation of B gold nanoparticles: 50mL 1.0mmol / LHAuCL 4 The aqueous s...

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Abstract

The invention discloses a biomolecular functionalized graphene / gold nano-particle composite film and a preparation method thereof, belonging to the technical field of inorganic film material. The composite film is obtained by self-assembly of graphene reduced and functionalized by bovine serum albumin and gold nano-particle electrostatic layer, wherein the chemical general formula is PEI / BSA-G- / (BSA-G+ / AuNPs)n; PEI is polyetherimide; BSA-G- is laminated bovine serum albumin functionalized graphene with negative charge; AuNPs is gold nano-particle; n is the layer number of multilayer film; and the thickness of the film is 15-500 nm. The laminated graphene with negative charge and gold nano-particles are assembled on a substrate repeatedly by electrostatic layer assembly technology to obtain an ordered and high-dispersion graphene / gold nano-particle three-dimensional composite film; and the instrumentation of the graphene material is achieved. The composite film can be widely applied in electro-catalysis and sensing field, and as a surface enhanced Raman substrate.

Description

technical field [0001] The invention belongs to the technical field of inorganic thin film materials, in particular to a biomolecule functionalized graphene / gold nanoparticle composite thin film and a preparation method. Background technique [0002] Graphene (Graphene, G) is a new type of two-dimensional planar nanomaterial discovered in 2004, which has a special single atomic layer structure. Due to the huge theoretical surface area and unique high conductivity of graphene, graphene and its composite materials have attractive application prospects in the fields of optics, electricity, and biosensing. There are many methods for preparing graphene, and the most widely used method for preparing graphene in large quantities is the reduction of graphene oxide with hydrazine hydrate. However, hydrazine hydrate is very toxic and pollutes the environment, which is not conducive to the mass production and wide application of graphene. In addition, there are still many problems in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08L79/08C08K13/06C08K9/04C08K3/04C08K3/08C08J5/18G01N27/30
Inventor 陈旭席倩杨文胜
Owner BEIJING UNIV OF CHEM TECH
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